Unfolded Protein Response is Involved in Trans-Platinum (II) Complex-Induced Apoptosis in Prostate Cancer Cells via ROS Accumulation

Basit Öğe Kaydı
dc.authoridDidem Karakaş Zeybek / 0000-0002-3781-6834
dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidDidem Karakaş Zeybek / 56422040600
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidDidem Karakaş Zeybek / L-6682-2018
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorKarakaş Zeybek, Didem
dc.contributor.authorCevatemre, Buse
dc.contributor.authorOral, Arzu Y.
dc.contributor.authorYılmaz, Veysel T.
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2020-08-30T20:07:03Z
dc.date.available2020-08-30T20:07:03Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.descriptionORAL, ARZU YILMAZTEPE/0000-0003-0463-6818; Karakas, Didem/0000-0002-3781-6834en_US
dc.description.abstractBackground: Prostate cancer is one of the most common cancer types and it is the sixth leading cause of cancer-related death in men worldwide. Even though novel treatment modalities have been developed, it still a lifethreatening disease. Therefore novel compounds are needed to improve the overall survival. Methods: In our study, it was aimed to evaluate the anti-cancer activity of newly synthesized Platinum (II) [Pt(II)] complex on DU145, LNCaP and PC-3 prostate cancer cell lines. The cytotoxic activity of Pt(II) complex was tested by SRB and ATP cell viability assays. To detect the mode of cell death; fluorescent staining, flow cytometry and western blot analyses were performed. Results: The Pt(II) complex treatment resulted in a decrease in cell viability and increasing levels of apoptotic markers (pyknotic nuclei, annexin-V, caspase 3/7 activity) and a decrease in mitochondrial membrane potential in a dose dependent manner. Among cell types, tested PC-3 cells were found to be more sensitive to Pt(II) complex, demonstrating elevation of DNA damage in this cell line. In addition, Pt(II) complex induced Endoplasmic Reticulum (ER) stress by triggering ROS generation. More importantly, pre-treatment with NAC alleviated Pt(II) complex-mediated ER stress and cell death in PC-3. Conclusion: These findings suggest an upstream role of ROS production in Pt(II) complex-induced ER stressmediated apoptotic cell death. Considering the ROS-mediated apoptosis inducing the effect of Pt(II) complex, it warrants further evaluation as a novel metal-containing anticancer drug candidate.en_US
dc.description.sponsorshipUludag University, Scientific Research ProjectsUludag University [OUAP(T)-2012/2]en_US
dc.description.sponsorshipThis study was supported by Uludag University, Scientific Research Projects (OUAP(T)-2012/2 coded project).en_US
dc.identifier.citationKarakas, D., Cevatemre, B., Oral, A. Y., Yilmaz, V. T., & Ulukaya, E. (2019). Unfolded Protein Response is Involved in Trans-Platinum (II) Complex-Induced Apoptosis in Prostate Cancer Cells via ROS Accumulation. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 19(9), 1184–1195. https://doi.org/10.2174/1871520619666190409103334en_US
dc.identifier.doi10.2174/1871520619666190409103334en_US
dc.identifier.endpage1195en_US
dc.identifier.issn1871-5206en_US
dc.identifier.issn1875-5992en_US
dc.identifier.issue9en_US
dc.identifier.pmid30963984en_US
dc.identifier.scopus2-s2.0-85074963173en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1184en_US
dc.identifier.urihttps://doi.org/10.2174/1871520619666190409103334
dc.identifier.urihttps://hdl.handle.net/20.500.12713/684
dc.identifier.volume19en_US
dc.identifier.wosWOS:000492373400010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarakaş Zeybek, Didemen_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofAnti-Cancer Agents In Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProstate Canceren_US
dc.subjectCytotoxicityen_US
dc.subjectApoptosisen_US
dc.subjectOxidative Stressen_US
dc.subjectUnfolded Protein Responseen_US
dc.subjectPlatinum(Ii) Complexen_US
dc.titleUnfolded Protein Response is Involved in Trans-Platinum (II) Complex-Induced Apoptosis in Prostate Cancer Cells via ROS Accumulationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
didem karakaş..pdf
Boyut:
2.24 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Moleküler Biyoloji ve Genetik Bölümü Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Makale Koleksiyonu