The detection of pomegranate (Punica granatum L.) peel apoptotic effects using AgNOR staining in MDA-MB-231

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dc.contributor.authorCeyhan, Rabia Nur
dc.contributor.authorNisari, Mustafa
dc.contributor.authorNisari, Mehtap
dc.contributor.authorUcar, Suemeyye
dc.contributor.authorKoca, Fatih Mehmet
dc.contributor.authorKerek, Gulderen
dc.contributor.authorOzcanli, Tugce
dc.date.accessioned2024-05-19T14:45:46Z
dc.date.available2024-05-19T14:45:46Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractIn present study, it was purposed to determine the in vitro effect of the extract obtained from the pomegranate (Punica granatum L.) peel on the breast cancer cell line. MDA-MB-231 cells were exposed to pomegranate peel extract (PoPx) at 37 degrees C and 5% CO2 for varying durations (24 and 48 h) and doses (25 and 50 mu g/mL). At the end of the incubation periods, argyrophilic nucleolus organizer regions (AgNOR) protein status, cell viability/apoptosis and cell cycle of MDA-MB-231 cells were examined in the Muse Cell Analyzer device. Cell viability was observed to be decreased when the groups treated with PoPx were compared with the control group. The group in which apoptosis was observed with the highest value was 50 mu g/mL PoPx group (p < 0.05). In the cell cycle test, the number of cells in the G0/G1 stage was found to be significantly higher in the 25 mu g/mL PoPx group compared to the control and 50 mu g/mL PoPx groups at the end of the 24-h incubation period (p < 0.05) The results also supported cell cycle and apoptosis, and at the end of 24 h, Total AgNOR area(TAA)/Total nuclear area (NA) ratio and AgNOR numbered decreased on the 50 mu g/mL PoPx group and were found to be statistically significant compared to the control group (p < 0.05). Consequently, it was determined that PoPx increased apoptosis on breast cancer cells by various mechanisms and inhibited cell viability/cell growth. This study showed that the widespread consumption of PoPx may be effective in preventing cancer formation and slowing its progression. [GRAPHICS] .en_US
dc.description.sponsorshipNuh Naci Yazgan University Scientific Research Projects Unit (BAP) [2021/SA-LTP-2]en_US
dc.description.sponsorshipThis study was supported by Nuh Naci Yazgan University Scientific Research Projects Unit (BAP) (Project code: 2021/SA-LTP-2).en_US
dc.identifier.doi10.1093/toxres/tfae015
dc.identifier.issn2045-452X
dc.identifier.issn2045-4538
dc.identifier.issue1en_US
dc.identifier.pmid38370142en_US
dc.identifier.scopus2-s2.0-85185448439en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org10.1093/toxres/tfae015
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5337
dc.identifier.volume13en_US
dc.identifier.wosWOS:001167119400014en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofToxicology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectMda-Mb-231en_US
dc.subjectPunica Granatum Len_US
dc.subjectAgnor Stainingen_US
dc.titleThe detection of pomegranate (Punica granatum L.) peel apoptotic effects using AgNOR staining in MDA-MB-231en_US
dc.typeArticleen_US

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