Serum Aspergillus galactomannan lateral flow assay for the diagnosis of invasive aspergillosis: a single center study

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dc.authoridMehmet Hilmi Doğu / 0000-0001-7237-2637
dc.authorscopusidMehmet Hilmi Doğu / 55212747300
dc.authorwosidMehmet Hilmi Doğu / W-2255-2017
dc.contributor.authorSerin, Istemi
dc.contributor.authorDoğu, Mehmet Hilmi
dc.date.accessioned2021-03-09T09:38:19Z
dc.date.available2021-03-09T09:38:19Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Aspergillus species meet the most important group of invasive fungal diseases (IFD) in immunosuppressed patients. Galactomannan is a polysaccharide antigen located in the wall structure of Aspergillus. The most commonly used method for antigen detection is enzymelinked immunoassay (ELISA). Aspergillus galactomannan Lateral Flow Assay (LFA) constitutes one of the new methods in the diagnosis of invasive aspergillosis (IA). The goal of this study is to demonstrate efficacy of LFA in our patients and to compare it to synchronous ELISA results. Methods: Galactomannan antigen was examined using both LFA and ELISA in serum samples taken from patients who were followed up in our hematology clinic. All patients are classified in subgroups as "proven", "probable", and “possible" patients according to the last EORTC / MSG guideline. Patients who met the “proven" IA criteria were included in the study as the gold standard. Results: A total of 87 patients were included in the study. Majority of patients had acute myeloid leukemia (AML) (56.3%). Eleven (12.6%) were in "proven" IA group. LFA test showed a superior diagnostic performance compared to ELISA (LFAAUC = 0.934 vs ELISAAUC = 0.545; p <0.001). The LFA had a sensitivity of 90.9%, and a specificity of 90.8% for "0.5 ODI" in predicting IA (PPV = 55.8%; NPV = 98.6%; p <0.001). Conclusion: The most important finding of this study is that the specificity of LFA was found to be higher for cut-off value of 0.5. It is recommended to combine the methods in many studies to provide a better early diagnosis for IA.en_US
dc.identifier.citationSerin, I., Dogu, M. H., (2021). Serum Aspergillus galactomannan Lateral Flow Assay for the Diagnosis of Invasive Aspergillosis: a single center study. Mycoses. https://doi.org/10.1111/myc.13265.en_US
dc.identifier.doi10.1111/myc.13265en_US
dc.identifier.issn1439-0507en_US
dc.identifier.pmid33683715en_US
dc.identifier.scopus2-s2.0-85102436014en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1111/myc.13265
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1525
dc.identifier.wosWOS:000628885000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDoğu, Mehmet Hilmi
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofMycosesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectInvasive Aspergillosisen_US
dc.subjectGalactomannanen_US
dc.subjectLateral Flow Assayen_US
dc.subjectELISAen_US
dc.subjectDiagnosisen_US
dc.titleSerum Aspergillus galactomannan lateral flow assay for the diagnosis of invasive aspergillosis: a single center studyen_US
dc.typeArticleen_US

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