Soloxolone methyl, as a 18?H-glycyrrhetinic acid derivate, may result in endoplasmic reticulum stress to induce apoptosis in breast cancer cells

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorAlper, Pınar
dc.contributor.authorSalomatina, Oksana V.
dc.contributor.authorSalakhutdinov, Nariman F.
dc.contributor.authorUlukaya, Engin
dc.contributor.authorArı, Ferda
dc.date.accessioned2021-01-04T08:36:01Z
dc.date.available2021-01-04T08:36:01Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBeing one of the leading causes of cancer death among women, various chemotherapeutic agents isolated from natural compounds are used in breast cancer treatment and consequently studies to develop new drugs still continue. There are several studies on 18?H-glycyrrhetinic acid, a secondary metabolite which is found in Glycyrrhiza glabra (liquorice roots), as a potential anticancer agent. In this study, the cytotoxic and apoptotic effects of Soloxolone methyl compound, a semisynthetic derivative of 18?H-glycyrrhetinic acid were investigated on breast cancer cells (MCF-7, MDA-MBA-231). Soloxolone methyl is found to be cytotoxic on both MCF-7 and MDA-MBA-231 breast cancer cells by inducing apoptosis. Especially in MDA-MB-231 cells apoptosis is detected to be triggered by ER stress. The antigrowth effects of Soloxolone methyl were determined using MTT and ATP assays. To identify the mode of cell death (apoptosis/necrosis), fluorescent staining (Hoechst 33342 and Propidium iodide) and caspase-cleaved cytokeratin 18 (M30-antigen) analyses were used. In addition, apoptosis was investigated on gene and protein levels by PCR and Western Blotting. Soloxolone methyl decreased cell viability on cells in a dose and time-dependent manner and induced apoptosis markers. An increase on apoptotic proteins related to endoplasmic reticulum stress (IRE1-?, Bip, CHOP) was also determined in MDA-MB-231 cells. Moreover, an increase of apoptotic gene expressions was determined in both cells treated with Soloxolone methyl. Advance analyses should be performed to elucidate the potential of Soloxolone methyl as an anticancer agent in breast cancer treatment.en_US
dc.identifier.citationAlper, P., Salomatina, O. V., Salakhutdinov, N. F., Ulukaya, E., & Ari, F. Soloxolone methyl, as a 18βH-glycyrrhetinic acid derivate, may result in endoplasmic reticulum stress to induce apoptosis in breast cancer cells. Bioorganic & Medicinal Chemistry, 30, 115963.en_US
dc.identifier.doi10.1016/j.bmc.2020.115963en_US
dc.identifier.issn0968-0896en_US
dc.identifier.pmid33383441en_US
dc.identifier.scopus2-s2.0-85098703773en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.bmc.2020.115963
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1298
dc.identifier.volume30en_US
dc.identifier.wosWOS:000612171500007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofBioorganic & Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject18βH-glycyrrhetinic Aciden_US
dc.subjectApoptosisen_US
dc.subjectBreast Canceren_US
dc.subjectER Stressen_US
dc.subjectSoloxolone Methylen_US
dc.titleSoloxolone methyl, as a 18?H-glycyrrhetinic acid derivate, may result in endoplasmic reticulum stress to induce apoptosis in breast cancer cellsen_US
dc.typeArticleen_US

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