LPS-Induced Neuron Cell Apoptosis through TNF-? and Cytochrome c Expression in Dental Pulp

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dc.contributor.authorSampoerno, G.
dc.contributor.authorSupriyanto, E.
dc.contributor.authorWahjuningrum, D.A.
dc.contributor.authorLarasati, A.A.
dc.contributor.authorArdiani, D.
dc.contributor.authorSurboyo, M.D.C.
dc.contributor.authorBhardwaj A.
dc.date.accessioned2024-05-19T14:33:21Z
dc.date.available2024-05-19T14:33:21Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractObjectives ?Inflammation of the dental pulp tissue caused by bacteria, creating an immunology response of death of the dental pulp, is called apoptosis. The Porphyromonas gingivalis that cause apoptosis is lipopolysaccharide (LPS) through toll-like receptor (TLR) via two different mechanisms, intracellular and extracellular pathways. This study analyzed the role of LPS exposure of neuron cells, tumor necrosis factor-? (TNF-?), and cytochrome c (cyt-c) expression in the dental pulp to predict the possible mechanism of apoptosis. Materials and Methods ?The lower tooth of Sprague Dawley rats was opened and exposed to LPS for 48 hours. Then the neuron cell analyzed histopathology using hematoxylin-eosin, whereas the TNF-? and cyt-c expression with indirect immunohistochemistry using a light microscope. The relationship between neuron cells with TNF-? and cyt-c was analyzed using stepwise regression linear analysis. Result ?The LPS exposure showed a lower number of neuron cells and had a relationship with TNF-? expression but not with cyt-c, while compared with control, both TNF-? and cyt-c expression were higher in neuron cells. Conclusion ?LPS exposure in dental pulp is possible to stimulate the apoptosis process through extracellular pathways marked by higher TNF-? expression. © 2023 Georg Thieme Verlag. All rights reserved.en_US
dc.identifier.doi10.1055/s-0043-1774329
dc.identifier.issn1305-7456
dc.identifier.scopus2-s2.0-85179692366en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1055/s-0043-1774329
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4201
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlagen_US
dc.relation.ispartofEuropean Journal of Dentistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectApoptosisen_US
dc.subjectCytochrome Cen_US
dc.subjectDental Pulpen_US
dc.subjectİmmunologyen_US
dc.subjectNeuron Cellen_US
dc.subjectTnf-?en_US
dc.titleLPS-Induced Neuron Cell Apoptosis through TNF-? and Cytochrome c Expression in Dental Pulpen_US
dc.typeArticleen_US

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