Genetic screening of the patients with primary immunodeficiency by whole-exome sequencing

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Küçük Resim

Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

New Rochelle

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Background: Primary immunodeficiencies (PIDs) are a heterogeneous group of congenital disorders characterized by susceptibility to recurrent infections, allergy, malignancies and autoimmunity. The identification of disease-causing genetic defects is critically important for treatment options. In last decade, next-generation sequencing (NGS)-based methods has enabled the rapid genetic screening and the discovery of new genetic defects in PIDs. In this study, we investigated causative mutations in patients with PID by NGS. Methods: We applied whole-exome sequencing in 8 PID patients. Detected mutations by NGS were validated by Sanger sequencing. Results: We made a genetic diagnosis in 5 of 8 (63%) patients, including 3 novel disease-causing variants. The identified mutations were found in RAG1, RAG2, JAK3, RFXANK, and CYBA genes. Conclusions: Our results show that whole-exome sequencing can facilitate the genetic diagnosis of the patients with PID.

Açıklama

Erman, Baran/0000-0001-9398-8465

Anahtar Kelimeler

Primary Immunodeficiency, Next-Generation Sequencing, Whole-Exome Sequencing

Kaynak

Pediatric Allergy Immunology and Pulmonology

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

1

Sayı

Künye

Erman, B., & Cipe, F. (2020). Genetic Screening of the Patients with Primary Immunodeficiency by Whole-Exome Sequencing. PEDIATRIC ALLERGY IMMUNOLOGY AND PULMONOLOGY, 33(1), 19–24. https://doi.org/10.1089/ped.2019.1097