İpek fibroin/polivinil alkol esaslı ilaç taşıyıcı yara örtüleri
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
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Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı, potansiyel yara örtüsü olarak ilaç taşıyıcı-yeni bir karboksil modifiyeli poli(vinil alkol)-çapraz bağlı ipek fibroin esaslı hidrojel filmi (İF/PVA) geliştirmektir. Çapraz bağlı hidrojellerin hazırlanması için poli(vinil alkol) (PVA),karboksil modifiye poli(vinil alkol) (PVA-COOH) verecek şekilde süksinik anhidrit (SA) ile modifiye edilmiş, FTIR ve 1H NMR analizleri ile karakterize edilmiştir. Takiben çözücü döküm yöntemi ile 1-etil-3-(3-dimetilaminopropil) karbodiimid (EDC) ve n-hidroksisüksinimid (NHS) varlığında farklı konsantrasyonlarda PVA-COOH ve ipek fibroin (İF) esaslı bir seri hidrojel filmler hazırlanmıştır. Elde edilen filmlerin yapıları FTIR ve XRD analizleri ile karakterize edilmiş, yüzey morfolojileri optik mikroskop altında incelenmiş, şişme davranışları ve mekaniksel dayanım özellikleri belirlenmiştir. Gerçekleştirilen sitotoksisitetestleri ile sentezlenen İF/PVA membran yara örtülerinin toksik olmadığı bulunmuştur. Optimum özelliklere sahip İF/PVA hidrojel filmine model ilaç olarak kuersetin yüklenmiş, etken madde yüklü filmlerin fosfat tamponu ortamında (pH:7,4) salım davranış profili incelenmiştir. Gerçekleştirilen in vitroyara modeli (çizik testi) sonuçları, 10 güne kadarkontrollü kuersetin salım profili gösteren İF/PVA filminin potansiyel ilaç taşıyıcı bir yara örtüsü olarak kullanılabileceğini göstermiştir.
The aim of this study was to develop a novel carboxyl modified poly(vinyl alcohol) - crosslinked silk fibroin based hydrogel film (IF/PVA) as a potential drug carrier wound dressing. For the preparation of crosslinked hydrogels, poly(vinyl alcohol) (PVA) was modified with succinic anhydride (SA) to give carboxyl modified poly(vinyl alcohol) (PVA-COOH) and characterized by FTIR and 1H NMR analyzes. Subsequently, a series of hydrogel films based on different concentrations of PVA-COOH and silk fibroin (IF) were prepared in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and nhydroxysuccinimide (NHS) by solvent casting method. The structures of the synthesized films were characterized by FTIR and XRD analyzes and their surface morphology was examined under optical microscope and their swelling behavior and mechanical strength properties were determined. According to the performed cytotoxicity tests, it was found that the synthesized IF/ PVA membrane dressings were not toxic. The selected IF/PVA hydrogel film with optimum properties was loaded with quercetin as a model drug and their release profile was investigated in phosphate buffered media (pH: 7.4). The results of the in vitro wound model (scratch test) have shown that IF/PVA film showing a controlled quercetin release profile over 10 days can be used as a potential drug-carrier wound dressing.
The aim of this study was to develop a novel carboxyl modified poly(vinyl alcohol) - crosslinked silk fibroin based hydrogel film (IF/PVA) as a potential drug carrier wound dressing. For the preparation of crosslinked hydrogels, poly(vinyl alcohol) (PVA) was modified with succinic anhydride (SA) to give carboxyl modified poly(vinyl alcohol) (PVA-COOH) and characterized by FTIR and 1H NMR analyzes. Subsequently, a series of hydrogel films based on different concentrations of PVA-COOH and silk fibroin (IF) were prepared in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and nhydroxysuccinimide (NHS) by solvent casting method. The structures of the synthesized films were characterized by FTIR and XRD analyzes and their surface morphology was examined under optical microscope and their swelling behavior and mechanical strength properties were determined. According to the performed cytotoxicity tests, it was found that the synthesized IF/ PVA membrane dressings were not toxic. The selected IF/PVA hydrogel film with optimum properties was loaded with quercetin as a model drug and their release profile was investigated in phosphate buffered media (pH: 7.4). The results of the in vitro wound model (scratch test) have shown that IF/PVA film showing a controlled quercetin release profile over 10 days can be used as a potential drug-carrier wound dressing.
Açıklama
Anahtar Kelimeler
İpek Fibroin, Polivinil Alkol, Yara Örtüsü, Hidrojel Film, Kontrollü Salım, Silk Fibroin, Polyvinyl Alcohol, Wound Dressing, Hydrogel Film, Controlled Release
Kaynak
Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
24
Sayı
1
Künye
ÖZTÜRK A. B,AKGÜNER Z. P (2020). İpek Fibroin/Polivinil Alkol Esaslı İlaç Taşıyıcı Yara Örtüleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 24(1), 25 - 34. Doi: 10.19113/sdufenbed.561177
