Isolation, culture, cryopreservation, and preparation of skin-derived fibroblasts as a final cellular product under good manufacturing practice-compliant conditions
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cell-based therapies have become a popular approach in the field of regenerative medicine. Human fibroblast cells, one of the cell types widely used in clinical applications, have been used for skin regeneration and wound healing procedures. Furthermore, they are utilized for aesthetic purposes since fibroblasts lose their abilities such as collagen synthesis with age. Here, we describe detailed procedures for isolation, culture, cryopreservation, and preparation of fibroblasts derived from adult human skin as a final product under good manufacturing practice-compliant conditions.
Açıklama
Anahtar Kelimeler
Cell Isolation, Cell Therapy, Culture, Fibroblasts, GMP
Kaynak
Methods in Molecular Biology
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
Sayı
Künye
Aydoğdu, N., Öztel, O. N., & Karaöz, E. (2020). Isolation, Culture, Cryopreservation, and Preparation of Skin-Derived Fibroblasts as a Final Cellular Product Under Good Manufacturing Practice-Compliant Conditions. Methods in molecular biology (Clifton, N.J.), 10.1007/7651_2020_333. Advance online publication. https://doi.org/10.1007/7651_2020_333
