Isolation, culture, cryopreservation, and preparation of skin-derived fibroblasts as a final cellular product under good manufacturing practice-compliant conditions

Küçük Resim Yok

Tarih

2021

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Cell-based therapies have become a popular approach in the field of regenerative medicine. Human fibroblast cells, one of the cell types widely used in clinical applications, have been used for skin regeneration and wound healing procedures. Furthermore, they are utilized for aesthetic purposes since fibroblasts lose their abilities such as collagen synthesis with age. Here, we describe detailed procedures for isolation, culture, cryopreservation, and preparation of fibroblasts derived from adult human skin as a final product under good manufacturing practice-compliant conditions.

Açıklama

Anahtar Kelimeler

Cell Isolation, Cell Therapy, Culture, Fibroblasts, GMP

Kaynak

Methods in Molecular Biology

WoS Q Değeri

N/A

Scopus Q Değeri

N/A

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Sayı

Künye

Aydoğdu, N., Öztel, O. N., & Karaöz, E. (2020). Isolation, Culture, Cryopreservation, and Preparation of Skin-Derived Fibroblasts as a Final Cellular Product Under Good Manufacturing Practice-Compliant Conditions. Methods in molecular biology (Clifton, N.J.), 10.1007/7651_2020_333. Advance online publication. https://doi.org/10.1007/7651_2020_333