Isolation, culture, cryopreservation, and preparation of skin-derived fibroblasts as a final cellular product under good manufacturing practice-compliant conditions

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorAydoğdu, Nurullah
dc.contributor.authorÖztel, Olga Nehir
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-12-24T13:13:09Z
dc.date.available2020-12-24T13:13:09Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractCell-based therapies have become a popular approach in the field of regenerative medicine. Human fibroblast cells, one of the cell types widely used in clinical applications, have been used for skin regeneration and wound healing procedures. Furthermore, they are utilized for aesthetic purposes since fibroblasts lose their abilities such as collagen synthesis with age. Here, we describe detailed procedures for isolation, culture, cryopreservation, and preparation of fibroblasts derived from adult human skin as a final product under good manufacturing practice-compliant conditions.en_US
dc.identifier.citationAydoğdu, N., Öztel, O. N., & Karaöz, E. (2020). Isolation, Culture, Cryopreservation, and Preparation of Skin-Derived Fibroblasts as a Final Cellular Product Under Good Manufacturing Practice-Compliant Conditions. Methods in molecular biology (Clifton, N.J.), 10.1007/7651_2020_333. Advance online publication. https://doi.org/10.1007/7651_2020_333en_US
dc.identifier.doi10.1007/7651_2020_333en_US
dc.identifier.pmid33349903en_US
dc.identifier.scopus2-s2.0-85103617705en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://www.doi.org/10.1007/7651_2020_333
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1289
dc.identifier.wosWOS:000683505000007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdal
dc.language.isoenen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell Isolationen_US
dc.subjectCell Therapyen_US
dc.subjectCultureen_US
dc.subjectFibroblastsen_US
dc.subjectGMPen_US
dc.titleIsolation, culture, cryopreservation, and preparation of skin-derived fibroblasts as a final cellular product under good manufacturing practice-compliant conditionsen_US
dc.typeArticleen_US

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