The effect of LDHs nanoparticles on the cellular behavior of stem cell-laden 3D-bioprinted scaffold
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SAGE PUBLICATIONS
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Three-dimensional (3D)-bioprinting as an emerging approach for tissue engineering possesses the promise to create highly mimicked organs or tissues by using computer-aided design. For biomedical applications in tissue engineering in our previous work, we developed an optimized nanocomposite bioink based on methylacrylated gelatin (GeIMA), methylacrylated chitosan (ChitMA), and double-layered hydroxide (LDHs) nanoparticles by using 3D-bioprinting technology. Herein, we used the previous formulation to fabricate human bone marrow mesenchymal stem cells (hBMMSCs)-laden nanocomposite bioinks. The effect of LDHs nanoparticles on the cellular behaviors of the encapsulated-hBMMSCs in the scaffolds was evaluated for the first time. Live/Dead, PrestoBlue, and DAPI/Actin analysis were carried out to assess the cell viability, proliferation rate, and cellular morphology of encapsulated hBMMSCs within the scaffolds. In addition, osteogenic differentiation studies were performed culturing the scaffolds for up to 21 days. Results show that LDHs nanoparticles in the GeIMA/ChitMA scaffold formulation increased the viability of hBMMSCs, did not cause any adverse effect on the proliferation rate, cell morphology of the hBMMSCs, and increased the Runx2 protein expression of the encapsulated-hBMMSCs in the scaffolds. This study progresses the LDHs containing nanocomposite bioink for cell printing applications in tissue engineering.
Açıklama
Anahtar Kelimeler
Bioink, Three-Dimensional-Bioprinting, Cell Laden, Double-Layered Hydroxide, Nanocomposite
Kaynak
JOURNAL OF BIOMATERIALS APPLICATIONS
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
Sayı
Künye
Izbudak, B., Bal-Ozturk, A. (2022). The effect of LDHs nanoparticles on the cellular behavior of stem cell-laden 3D-bioprinted scaffold. Journal of Biomaterials Applications.
