The effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injury

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dc.authoridTuna Bilecik / 0000-0002-7160-2127en_US
dc.authorscopusidTuna Bilecik / 55758337600
dc.authorwosidTuna Bilecik / ELH-9331-2022
dc.contributor.authorBilecik, Tuna
dc.contributor.authorKarateke, F.
dc.contributor.authorElkan, H.
dc.contributor.authorGokce, H.
dc.date.accessioned2020-08-30T20:06:51Z
dc.date.available2020-08-30T20:06:51Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractOBJECTIVE: Mammalian transient receptor potential melastatin (TRPM) channels are a form of calcium channels and they transport calcium and magnesium ions. TRPM has eight subclasses including TRPM1-8. TRPM2, TRPM6, TRPM7, TRPM8 are expressed especially in the liver cell. Therefore, we aim to investigate the effects of TRPM2, TRPM6, TRPM7, and TRPM8 gene expression and histopathologic changes after treatment of verapamil in the hepatic ischemia-reperfusion rat model. MATERIALS AND METHODS: Animals were randomly assigned to one or the other of the following groups including sham (n=8) group, verapamil (calcium entry blocker) (n=8) group, I/R group (n=8) and I/R-verapamil (n=8) group. TRPM 2, 6, 7, 8 gene expression level was assessed by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) and histopathologic changes were determined by the hematoxylin and eosin (HE) examination. RESULTS: The expression level of TRPM 2, 6, 7, and 8 genes was significantly higher in ischemia- reperfusion (I/R), verapamil, IR-verapamil groups compared to sham group. The p-values were 0.0024, < 0.0001, 0.0002, 0.006 for TRPM2, TRPM6, TRPM7, and TRPM8, respectively. Severe necrotic, degenerative differentiations and severe hemorrhagic areas were observed in hepatocytes from IR group. Also, moderate necrotic and degenerative differentiations and moderate hemorrhagic areas were observed in hepatocytes from IR-verapamil group. CONCLUSIONS: This is the first study reporting an association between the expression level of TRPM 2, 6, 7, 8 in a hepatic ischemia-reperfusion rat model. Moreover, TRPM 2, 6, 7, 8 affect hepatic ischemia-reperfusion.en_US
dc.identifier.citationBilecik, T., Karateke, F., Elkan, H., & Gokce, H. (2019). The effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injury. European review for medical and pharmacological sciences, 23(7), 3088-3095.en_US
dc.identifier.doi10.26355/eurrev_201904_17592en_US
dc.identifier.endpage3095en_US
dc.identifier.issn1128-3602en_US
dc.identifier.issue7en_US
dc.identifier.pmid31002158en_US
dc.identifier.scopus2-s2.0-85064381864en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage3088en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/639
dc.identifier.urihttps://doi.org/10.26355/eurrev_201904_17592en_US
dc.identifier.volume23en_US
dc.identifier.wosWOS:000466386900047en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBilecik, Tunaen_US
dc.language.isoenen_US
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIschemiaen_US
dc.subjectReperfusionen_US
dc.subjectTrpen_US
dc.subjectTrpmen_US
dc.subjectVerapamilen_US
dc.subjectCalcium Entry Blockeren_US
dc.titleThe effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injuryen_US
dc.typeArticleen_US

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