Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Int Inst Anticancer Research
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: The inhibition of autophagy using pharmacological inhibitors such as chloroquine may be an effective strategy to overcome chemotherapy or resistance to anti-angiogenic therapy. Materials and Methods: The cytotoxic effect of doxorubicin (0.1-1 mu M), chloroquine (0.25-32 ,mu M) and their combination were investigated by employing ATP assay in human umbilical vein endothelial cells (HUVECs). The effect of doxorubicin and chloroquine combination was also measured using tube formation assay on Matrigel. The anti-angiogenic activities of doxorubicin (2.5 mu g/pellet) and chloroquine (15 mu g/pellet), their combination, and standards (50 mu g/pellet) were tested in vivo using the chick embryo chorioallantoic membrane (CAM) assay. Results: The combination of doxorubicin and chloroquine significantly had a stronger anti-angiogenic effect than the positive control (+/-)-thalidomide and doxorubicin alone in the CAM assay and in vitro tube-formation assay. Conclusion: Chloroquine enhanced the anti-angiogenic effect of doxorubicin on CAM at the tested concentrations.
Açıklama
Anahtar Kelimeler
Doxorubicin, Chloroquine, Angiogenesis, Cam Assay, Tube Formation
Kaynak
Anticancer Research
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
38
Sayı
7
Künye
Gurel-Gurevin, E., Kiyan, H. T., Esener, O. B. B., Aydinlik, S., Uvez, A., Ulukaya, E., ... & Armutak, E. I. (2018). Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo. Anticancer research, 38(7), 4011-4020.
