Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorGürel-Gürevin, Ebru
dc.contributor.authorKıyan, Hülya Tuba
dc.contributor.authorEsener, Osman Behzat Burak
dc.contributor.authorAydınlık, Şeyma
dc.contributor.authorÜvez, Ayça
dc.contributor.authorUlukaya, Engin
dc.contributor.authorDimas, Konstantinos
dc.contributor.authorArmutak, Elif İlkay
dc.date.accessioned2020-08-30T20:07:30Z
dc.date.available2020-08-30T20:07:30Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: The inhibition of autophagy using pharmacological inhibitors such as chloroquine may be an effective strategy to overcome chemotherapy or resistance to anti-angiogenic therapy. Materials and Methods: The cytotoxic effect of doxorubicin (0.1-1 mu M), chloroquine (0.25-32 ,mu M) and their combination were investigated by employing ATP assay in human umbilical vein endothelial cells (HUVECs). The effect of doxorubicin and chloroquine combination was also measured using tube formation assay on Matrigel. The anti-angiogenic activities of doxorubicin (2.5 mu g/pellet) and chloroquine (15 mu g/pellet), their combination, and standards (50 mu g/pellet) were tested in vivo using the chick embryo chorioallantoic membrane (CAM) assay. Results: The combination of doxorubicin and chloroquine significantly had a stronger anti-angiogenic effect than the positive control (+/-)-thalidomide and doxorubicin alone in the CAM assay and in vitro tube-formation assay. Conclusion: Chloroquine enhanced the anti-angiogenic effect of doxorubicin on CAM at the tested concentrations.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul UniversityIstanbul University [49841, 55089, 21160, 20856, 49997, 53382]en_US
dc.description.sponsorshipThe Authors would like to thank especially Mrs. Emine Kircali Kocabas from Has Tavuk Company for providing the fertilized hen eggs. This work was supported by Scientific Research Project Coordination Unit of Istanbul University. Project numbers: 49841, 55089, 21160, 20856, 49997 and 53382.en_US
dc.identifier.citationGurel-Gurevin, E., Kiyan, H. T., Esener, O. B. B., Aydinlik, S., Uvez, A., Ulukaya, E., ... & Armutak, E. I. (2018). Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo. Anticancer research, 38(7), 4011-4020.en_US
dc.identifier.doi10.21873/anticanres.12689en_US
dc.identifier.endpage4020en_US
dc.identifier.issn0250-7005en_US
dc.identifier.issn1791-7530en_US
dc.identifier.issue7en_US
dc.identifier.pmid29970525en_US
dc.identifier.scopus2-s2.0-85049800874en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage4011en_US
dc.identifier.urihttps://doi.org/10.21873/anticanres.12689
dc.identifier.urihttps://hdl.handle.net/20.500.12713/776
dc.identifier.volume38en_US
dc.identifier.wosWOS:000449943500028en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherInt Inst Anticancer Researchen_US
dc.relation.ispartofAnticancer Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDoxorubicinen_US
dc.subjectChloroquineen_US
dc.subjectAngiogenesisen_US
dc.subjectCam Assayen_US
dc.subjectTube Formationen_US
dc.titleChloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivoen_US
dc.typeArticleen_US

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