Embryonic aortic arch material properties obtained by optical coherence tomography-guided micropipette aspiration
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
It is challenging to determine the in vivo material properties of a very soft, mesoscale arterial vesselsof size ? 80 to 120 ?m diameter. This information is essential to understand the early embryonic cardiovascular development featuring rapidly evolving dynamic microstructure. Previous research efforts to describe the properties of the embryonic great vessels are very limited. Our objective is to measure the local material properties of pharyngeal aortic arch tissue of the chick-embryo during the early Hamburger-Hamilton (HH) stages, HH18 and HH24. Integrating the micropipette aspiration technique with optical coherence tomography (OCT) imaging, a clear vision of the aspirated arch geometry is achieved for an inner pipette radius of Rp = 25 ?m. The aspiration of this region is performed through a calibrated negatively pressurized micro-pipette. A computational finite element model is developed to model the nonlinear behaviour of the arch structure by considering the geometry-dependent constraints. Numerical estimations of the nonlinear material parameters for aortic arch samples are presented. The exponential material nonlinearity parameter (a) of aortic arch tissue increases statistically significantly from a = 0.068 ± 0.013 at HH18 to a = 0.260 ± 0.014 at HH24 (p = 0.0286). As such, the aspirated tissue length decreases from 53 ?m at HH18 to 34 ?m at HH24. The calculated NeoHookean shear modulus increases from 51 Pa at HH18 to 93 Pa at HH24 which indicates a statistically significant stiffness increase. These changes are due to the dynamic changes of collagen and elastin content in the media layer of the vessel during development. © 2022 Elsevier Ltd
Açıklama
Anahtar Kelimeler
Biaxial Testing, Chick Embryo, Finite Element, Hyperelastic Models, Mechanical Contact, Mechanotransduction, Pharyngeal Aortic Arch, Pipette Aspiration, Soft Tissue
Kaynak
Journal of Biomechanics
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
146
Sayı
Künye
Lashkarinia, S. S., Coban, G., Siddiqui, H. B., Yap, C. H., & Pekkan, K. (2022). Embryonic aortic arch material properties obtained by optical coherence tomography-guided micropipette aspiration. Journal of Biomechanics, 111392.
