Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer

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dc.authoridÇakır Gündoğdu, Ayşe/0000-0002-2466-9417
dc.authorwosidÇakır Gündoğdu, Ayşe/ABE-4977-2020
dc.contributor.authorOzyurt, Rumeysa
dc.contributor.authorErkasap, Nilufer
dc.contributor.authorOzkurt, Mete
dc.contributor.authorErkasap, Serdar Mustafa
dc.contributor.authorDimas, Konstantinos
dc.contributor.authorGundogdu, Ayse Cakir
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2024-05-19T14:40:15Z
dc.date.available2024-05-19T14:40:15Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackground/aim: Colorectal cancer (CRC) is a fatal malignancy type and its occurence still needs to be explored mechanistically. Notch, IL-1, and leptin crosstalk is reported to play a role in the proliferation, migration, and expression of proangiogenic molecules. In this study, we aimed to investigate the effect of inhibition of Notch, IL-1, and leptin on CRC.Materials and methods: To generate colorectal cancer tumor xenografts, 1 x 107 cells from exponentially growing cultures of HCT-15 cells were injected subcutaneously, at the axillary region of the left and right rear flanks of forty NOD.CB17-Prkdcscid/J (NOD/SCID) female mice. The mice were then monitored for the development of tumors and were randomly divided into five groups when tumor sizes reached a volume of approximately 150 mm3. Mice were used to determine the effectiveness of the gamma-secretase inhibitor (DAPT, Notch inhibitor), the interleukin-1 receptor antagonist (Anakinra) and the leptin receptor antagonist (Allo aca) against tumor growth. The mice were euthanized by CO2 inhalation immediately after the treatments finished, and all efforts were made to minimize suffering. Tumors were excissed for RT-PCR and histological analysis.Results: There is an intact Notch, IL-1, and leptin signaling axis, and in vivo antagonism of Notch, IL-1, and leptin affects mRNA and protein expression of inflammatory and angiogenic molecules.Conclusion: Present data suggest that targeting Notch, IL-1, and leptin may be possesses therapeutic potential in CRC.en_US
dc.description.sponsorshipEskisehir Osmangazi University Scientific Research Projects Committee [201711028]en_US
dc.description.sponsorshipAll funding for this study was supported by a grant from Eskisehir Osmangazi University Scientific Research Projects Committee (no: 201711028), Tuerkiye. No additional external funding was received for this study.en_US
dc.identifier.doi10.55730/1300-0152.2663
dc.identifier.endpage300en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4en_US
dc.identifier.pmid38152619en_US
dc.identifier.scopus2-s2.0-85171875114en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage290en_US
dc.identifier.trdizinid1208208en_US
dc.identifier.urihttps://doi.org10.55730/1300-0152.2663
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1208208
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4930
dc.identifier.volume47en_US
dc.identifier.wosWOS:001085260400006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectColorectal Canceren_US
dc.subjectNotchen_US
dc.subjectIl-1en_US
dc.subjectLeptinen_US
dc.subjectProinflammatoryen_US
dc.subjectProangiogenicen_US
dc.titleTargeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal canceren_US
dc.typeArticleen_US

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