Enzyme-mediated Alleviation of Peroxide Toxicity in Self-oxygenating Biomaterials

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dc.authoridAyça Bal Öztürk / 0000-0002-6502-528Xen_US
dc.authorscopusidAyça Bal Öztürk / 57062000100
dc.authorwosidAyça Bal Öztürk / M-4472-2018
dc.contributor.authorWillemen, Niels G. A.
dc.contributor.authorHassan, Shabir
dc.contributor.authorGurian, Melvin
dc.contributor.authorJasso-Salazar, Maria Fernanda
dc.contributor.authorFan, Kai
dc.contributor.authorWang, Haihang
dc.contributor.authorBecker, Malin
dc.contributor.authorAllijn, Iris E.
dc.contributor.authorBal Öztürk, Ayça
dc.contributor.authorLeijten, Jeroen
dc.contributor.authorShin, Su Ryon
dc.date.accessioned2022-04-06T05:47:00Z
dc.date.available2022-04-06T05:47:00Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Temel Bilimleri Bölümüen_US
dc.description.abstractOxygen releasing biomaterials can facilitate the survival of living implants by creating environments with a viable oxygen level. Hydrophobic oxygen generating microparticles (HOGMPs) encapsulated calcium peroxide (CPO) have recently been used in tissue engineering to release physiologically relevant amounts of oxygen for several weeks. However, generating oxygen using CPO is mediated via the generation of toxic levels of hydrogen peroxide (H2 O2 ). The incorporation of antioxidants, such as catalases, could potentially reduce H2 O2 levels. However, the formulation in which catalases can most effectively scavenge H2 O2 within oxygen generating biomaterials has remained unexplored. In this study, four distinct catalase incorporation methods were compared based on their ability to decrease H2 O2 levels. Specifically, catalase was incorporated within HOGMPs, or absorbed onto HOGMPs, or freely laden into the hydrogel entrapping HOGMPs and compared with control without catalase. Supplementation of free catalase in an HOGMP-laden hydrogel significantly decreased H2 O2 levels reflecting a higher cellular viability and metabolic activity of all the groups. An HOGMP/catalase-laden hydrogel precursor solution containing cells was used as an oxygenating bioink allowing improved viability of printed constructs under severe hypoxic conditions. The combination of HOGMPs with a catalase-laden hydrogel has the potential to decrease peroxide toxicity of oxygen generating tissues. This article is protected by copyright. All rights reserved.en_US
dc.identifier.citationWillemen, N. G., Hassan, S., Gurian, M., Jasso‐Salazar, M. F., Fan, K., Wang, H., ... & Shin, S. R. Enzyme‐mediated Alleviation of Peroxide Toxicity in Self‐oxygenating Biomaterials. Advanced Healthcare Materials, 2102697.en_US
dc.identifier.doi10.1002/adhm.202102697en_US
dc.identifier.pmid35362224en_US
dc.identifier.scopus2-s2.0-85128066052en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1002/adhm.202102697
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2619
dc.identifier.wosWOS:000782668700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBal Öztürk, Ayça
dc.language.isoenen_US
dc.relation.ispartofAdv Healthc Materen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3D Bioprintingen_US
dc.subjectCatalaseen_US
dc.subjectOxygen Generationen_US
dc.subjectPeroxide Toxicityen_US
dc.subjectTissue Engineeringen_US
dc.titleEnzyme-mediated Alleviation of Peroxide Toxicity in Self-oxygenating Biomaterialsen_US
dc.typeArticleen_US

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