Soloxolone methyl induces apoptosis and oxidative/ER stress in breast cancer cells and target cancer stem cell population

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dc.authoridakgün, oğuzhan/0000-0002-8410-1786;
dc.authorwosidakgün, oğuzhan/A-5608-2019
dc.authorwosidErtürk, Elif/AAK-3371-2021
dc.authorwosidErturk, Elif/JQI-3400-2023
dc.contributor.authorErturk, Elif
dc.contributor.authorAkgun, Oguzhan
dc.contributor.authorYildiz, Yaren
dc.contributor.authorKalkan, Pinar A. L. P. E. R.
dc.contributor.authorSalomatina, Oksana V.
dc.contributor.authorSalakhutdinov, Nariman F.
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2024-05-19T14:45:45Z
dc.date.available2024-05-19T14:45:45Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractOne of the most prevalent malignancies in women and one of the leading causes of cancer-related death is breast cancer. There is a need for new treatment approaches and drugs for breast cancer. Many studies show the high potential of triterpene compounds and their semisynthetic derivatives as anticancer agents due to their ability to induce apoptosis and suppress tumorigenesis. The effects of soloxolone methyl (SM), a semisynthetic derivative of 18-H-glycyrrhetinic acid, on the cytotoxicity and apoptosis of human breast cancer cell line (T-47D) and cancer stem cell (CSCs) population (mammospheres; CD44+/CD24-antigen) derived from breast cancer cells, were examined in this work. The ATP assay was used to determine SM growth-inhibitory effects. Fluorescent staining, caspase-cleaved cytokeratin 18, and flow cytometry analysis were used to determine the mode of the cell death. In addition, cell death was investigated at protein and gene levels by Western Blotting and PCR, respectively. SM resulted in cytotoxicity in a time and dose dependent manner via ROS production and ER stress in T-47D cells in 2 models. The mode of cell death was apoptosis, evidenced by phosphatidylserine exposure, caspase activation, and bax overexpression. In mammospheres as 3D model, SM decreased stem cell properties and induced cell death. Taken together, SM may be a promising agent in the treatment of breast cancer, especially due to its antigrowth activity on CSCs.en_US
dc.description.sponsorshipBursa Uludag University Scientific Research Projects Coordination Unit [TGA-2021-513]; Council of Higher Education (YOK) PhD Scholarship Program [100/2000]en_US
dc.description.sponsorshipThis work was supported by the Bursa Uludag University Scientific Research Projects Coordination Unit for the project that is numbered TGA-2021-513. Oguzhan AKGUEN is a PhD student financed by the Council of Higher Education (YOK) 100/2000 PhD Scholarship Program.en_US
dc.identifier.doi10.55730/1300-0152.2660
dc.identifier.endpage261en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4en_US
dc.identifier.pmid38152618en_US
dc.identifier.scopus2-s2.0-85171863352en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage247en_US
dc.identifier.trdizinid1208205en_US
dc.identifier.urihttps://doi.org10.55730/1300-0152.2660
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1208205
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5331
dc.identifier.volume47en_US
dc.identifier.wosWOS:001085260400003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectMammosphereen_US
dc.subjectSoloxolone Methylen_US
dc.subjectApoptosisen_US
dc.subjectBreast Canceren_US
dc.titleSoloxolone methyl induces apoptosis and oxidative/ER stress in breast cancer cells and target cancer stem cell populationen_US
dc.typeArticleen_US

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