Investigation of possible associations between tryptophan/kynurenine status and FOXP3 expression in colorectal cancer

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dc.authoridEbru Nur Ay / 0000-0001-7121-4362en_US
dc.authorscopusidEbru Nur Ay / 57209852424
dc.authorwosidEbru Nur Ay / L-5910-2018en_US
dc.contributor.authorAy, Ebru Nur
dc.contributor.authorHakan, Mehmet Tolgahan
dc.contributor.authorArıkan, Soykan
dc.contributor.authorDemirkol, Şeyda
dc.contributor.authorDoğan, Mehmet Baki
dc.contributor.authorHepokur, Ceylan Özsoy
dc.contributor.authorYaylım, İlhan
dc.contributor.authorAkyüz, Filiz
dc.contributor.authorHorozoğlu, Cem
dc.date.accessioned2022-05-20T11:43:37Z
dc.date.available2022-05-20T11:43:37Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractTryptophan metabolism in the tumor microenvironment exerts immunosuppressive effects by affecting the anti-tumor functions of immune cells. The immunosuppressive roles of tryptophan and tryptophan metabolites and their effects on the FOXP3 gene, highly expressed in regulatory T cells (Tregs), are remarkable. Our study aimed to investigate the relation between tryptophan metabolism and the transcription factor FOXP3 gene in colorectal cancer (CRC). Patients with CRC (n?=?159) and controls (n?=?112) were included in the study. The FOXP3 rs3761548 variant genotyping from the isolated genomic DNA was performed by PCR-RFLP. FOXP3 gene expression was determined by Q-PCR in RNAs isolated from resected tissues at the same time. Serum tryptophan, kynurenine, kynurenic acid levels of the cases were determined by HPLC. In serum samples with CRC, tryptophan level was 14.32?±?1.09??mol/L, kynurenine level was 1.33?±?0.02??mol/L, and the kynurenic acid level was 0.01?±?0.001??mol/L. The level of tryptophan was found to be low in CRC compared to control (p?<?.001). In cases with CRC, CC genotype (p?=?.048) and C allele (p?=?.012) frequency for FOXP3 rs3761548 were higher than the control group. It was found that the expression level of the FOXP3 gene was approximately 44 times higher in the advanced tumor stage (T3?+?T4) than in the early tumor stage (T1?+?T2) (p?=?.021). We suggest that there may be a possible relationship among serum TRP, TRP metabolites (KYN, KYNA) levels, FOXP3 gene expression, and FOXP3 gene variants in CRC pathogenesis.en_US
dc.identifier.citationAy EN, Demirkol Ş, Hakan MT, Horozoğlu C, Arıkan S, Doğan MB, Akyüz F, Hepokur CÖ, Yaylım İ. Investigation of possible associations between tryptophan/kynurenine status and FOXP3 expression in colorectal cancer. Scand J Clin Lab Invest. 2022 May;82(3):185-191. doi: 10.1080/00365513.2022.2040050. Epub 2022 Apr 22. PMID: 35452343.en_US
dc.identifier.doi10.1080/00365513.2022.2040050en_US
dc.identifier.endpage191en_US
dc.identifier.issn0036-5513en_US
dc.identifier.issue3en_US
dc.identifier.pmid35452343en_US
dc.identifier.scopus2-s2.0-85129639458en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage185en_US
dc.identifier.urihttp://doi.org/10.1080/00365513.2022.2040050
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2693
dc.identifier.volume82en_US
dc.identifier.wosWOS:000785876400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAy, Ebru Nur
dc.language.isoenen_US
dc.publisherTaylor and Francisen_US
dc.relation.ispartofScandinavian Journal of Clinical and Loboratory İnvestigationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPCR-RFLPen_US
dc.subjectHPLCen_US
dc.subjectColorectal Cancer (CRC)en_US
dc.subjectTryptophan Metabolisen_US
dc.subjectGene Expressionen_US
dc.titleInvestigation of possible associations between tryptophan/kynurenine status and FOXP3 expression in colorectal canceren_US
dc.typeArticleen_US

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