Evaluation of neurogranin levels in a rat model of diffuse axonal injury

Küçük Resim Yok

Tarih

2024

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Nencki Inst Experimental Biology

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Diffuse axonal injury (DAI), one of the most common and devastating type of traumatic brain injury, is the result of the shear force on axons due to severe rotational acceleration and deceleration. Neurogranin (NRGN) is a postsynaptic protein secreted by excitatory neurons, and synaptic dysfunction can alter extracellular NRGN levels. In this study, we examined NRGN levels in serum and cerebrospinal fluid (CSF) after experimental DAI in terms of their diagnostic value. Experimental DAI was induced using the Marmarou technique in male Wistar albino rats. Serum and CSF NRGN levels of the sham group, one-hour, six-hour, 24-hour, and 72-hour post-DAI groups were measured by ELISA method. DAI was verified by staining with hematoxylin-eosin and beta-amyloid precursor protein in the rat brain samples. While no histopathological and immunohistochemical changes were observed in the early hours of the post-DAI groups, the staining of the beta-APP visibly increased over time, with positivity being most frequent and intense in the 72-hour group. It was found that serum NRGN levels were significantly lower in the 6-hour group than in the sham group. The serum NRGN levels in the 24-hour group were significantly higher than those in the sham group. This study showed a dichotomy of post-DAI serum NRGN levels in consecutive time periods. NRGN levels in CSF were higher in the one-hour group than in the sham group and returned to baseline by 72 hours, although not significantly. Our study provides an impression of serum and CSF NRGN levels in a rat DAI model in consecutive time periods. Further studies are needed to understand the diagnostic value of NRGN.

Açıklama

Anahtar Kelimeler

Axonal Damage, Diffuse Axonal Injury, Neurogranin, Rat Model, Traumatic Brain Injury

Kaynak

Acta Neurobiologiae Experimentalis

WoS Q Değeri

N/A

Scopus Q Değeri

Q3

Cilt

84

Sayı

1

Künye