Evaluation of neurogranin levels in a rat model of diffuse axonal injury

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dc.contributor.authorBaskurt, Ozan
dc.contributor.authorAydin, Seckin
dc.contributor.authorAvci, Idris
dc.contributor.authorNayir, Pinar Ozay
dc.contributor.authorAydin, Mehmet Volkan
dc.date.accessioned2024-05-19T14:39:14Z
dc.date.available2024-05-19T14:39:14Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractDiffuse axonal injury (DAI), one of the most common and devastating type of traumatic brain injury, is the result of the shear force on axons due to severe rotational acceleration and deceleration. Neurogranin (NRGN) is a postsynaptic protein secreted by excitatory neurons, and synaptic dysfunction can alter extracellular NRGN levels. In this study, we examined NRGN levels in serum and cerebrospinal fluid (CSF) after experimental DAI in terms of their diagnostic value. Experimental DAI was induced using the Marmarou technique in male Wistar albino rats. Serum and CSF NRGN levels of the sham group, one-hour, six-hour, 24-hour, and 72-hour post-DAI groups were measured by ELISA method. DAI was verified by staining with hematoxylin-eosin and beta-amyloid precursor protein in the rat brain samples. While no histopathological and immunohistochemical changes were observed in the early hours of the post-DAI groups, the staining of the beta-APP visibly increased over time, with positivity being most frequent and intense in the 72-hour group. It was found that serum NRGN levels were significantly lower in the 6-hour group than in the sham group. The serum NRGN levels in the 24-hour group were significantly higher than those in the sham group. This study showed a dichotomy of post-DAI serum NRGN levels in consecutive time periods. NRGN levels in CSF were higher in the one-hour group than in the sham group and returned to baseline by 72 hours, although not significantly. Our study provides an impression of serum and CSF NRGN levels in a rat DAI model in consecutive time periods. Further studies are needed to understand the diagnostic value of NRGN.en_US
dc.description.sponsorshipTurkish Neurosurgical Societyen_US
dc.description.sponsorshipPreparation for publication of this article is partly supported by Turkish Neurosurgical Society and Prof. Dr. Cemil Tascioglu State Hospital Medical Expertise Education Board.en_US
dc.identifier.doi10.55782/ane-2024-2543
dc.identifier.endpage88en_US
dc.identifier.issn0065-1400
dc.identifier.issn1689-0035
dc.identifier.issue1en_US
dc.identifier.pmid38587321en_US
dc.identifier.scopus2-s2.0-85190399512en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage80en_US
dc.identifier.urihttps://doi.org10.55782/ane-2024-2543
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4733
dc.identifier.volume84en_US
dc.identifier.wosWOS:001203095500008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherNencki Inst Experimental Biologyen_US
dc.relation.ispartofActa Neurobiologiae Experimentalisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAxonal Damageen_US
dc.subjectDiffuse Axonal Injuryen_US
dc.subjectNeurograninen_US
dc.subjectRat Modelen_US
dc.subjectTraumatic Brain Injuryen_US
dc.titleEvaluation of neurogranin levels in a rat model of diffuse axonal injuryen_US
dc.typeArticleen_US

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