Metagenomic analysis of black-stained plaques in permanent dentition

Basit Öğe Kaydı
dc.authoridHalil Çelik / 0000-0002-0732-2403
dc.authoridZeynep Ceren Çelik / 0000-0002-6900-2632
dc.authorscopusidHalil Çelik / 57224070089
dc.authorscopusidZeynep Ceren Çelik / 57216658409
dc.authorwosidHalil Çelik / CKT-9433-2022
dc.authorwosidZeynep Ceren Çelik / AAW-9821-2021
dc.contributor.authorÇelik, Zeynep Ceren
dc.contributor.authorÇakiris, Aris
dc.contributor.authorYanıkoğlu, Funda Çalışkan
dc.contributor.authorAbacı, Neslihan
dc.contributor.authorEkmekçi, Sema Sırma
dc.contributor.authorIlgın, Can
dc.contributor.authorÇelik, Halil
dc.contributor.authorTa?tekin, Dilek Arslantunalı
dc.date.accessioned2021-06-08T11:19:58Z
dc.date.available2021-06-08T11:19:58Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractObjectives: We aimed to determine the aetiologic agent responsible for black staining of permanent dentition using next-generation sequencing and determine the relationship between caries and black stains. Materials and methods: A total of 52 systemically healthy patients with black-stained and caries-free (n = 13), black-stained and carious (n = 13), black stain-free and caries-free (n = 13), and black stain-free and carious (n = 13) teeth were enrolled in the study. The International Caries Detection and Assessment System (ICDAS II) was used for caries classification. Between 08:00 and 10:00, supragingival plaque samples were collected after a minimum of 8–12 h of accumulation and DNA samples were isolated. The samples were processed using the ZymoBIOMICS™ Service. Bioinformatics analysis was performed using mothur at usegalaxy.org. Data were analysed statistically using the Pearson chi-square and Fisher tests. Results: The number of caries-free teeth (ICDAS 0, 1, and 2) was significantly higher in patients with black stains (p = 0.007).Capnocytophaga (4.8 %), Corynebacterium (3.9 %), and Neisseria (5.4 %) species were the most abundant among all black-stained plaques (carious and caries-free) (p < 0.05). Capnocytophaga (10.8 %), Cardiobacterium (3.6 %), and Rothia (1.72 %) species were detected in the black-stained plaques of caries-free patients (p < 0.05). Conclusion: This study is one of the first studies examining the microbial composition of dental plaques with black staining in carious and caries-free adult patients using next generation sequencing technology. In the presence of black staining, plaques have an ultimate complex microbial structure. A lower caries burden was noted in the presence of black staining.en_US
dc.identifier.citationÇelik, Z. C., Çakiris, A., Yanıkoğlu, F., Abacı, N., Ekmekçi, S. S., Ilgın, C., ... & Tağtekin, D. (2021). Metagenomic analysis of black-stained plaques in permanent dentition. Archives of Oral Biology, 105171.en_US
dc.identifier.doi10.1016/j.archoralbio.2021.105171en_US
dc.identifier.issn0003-9969en_US
dc.identifier.pmid34058724en_US
dc.identifier.scopus2-s2.0-85106869404en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2021.105171
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1788
dc.identifier.volume128en_US
dc.identifier.wosWOS:000659789300008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÇelik, Zeynep Ceren
dc.institutionauthorÇelik, Halil
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofArchives of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBlack Stainen_US
dc.subjectDental Cariesen_US
dc.subjectNext Generation Sequencingen_US
dc.subjectOral Microbiomeen_US
dc.titleMetagenomic analysis of black-stained plaques in permanent dentitionen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
65.pdf
Boyut:
4.38 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Klinik Bilimler Bölümü Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu