Comparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection: an experimental study

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Küçük Resim

Tarih

2021

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Wolters Kluwer

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

This study was designed to evaluate the efficacy of epineural tubulization (ENT) with or without intratubal application of adipose-derived mesenchymal stem cells (ASCs) in the rat model of sciatic nerve transection. After formation of 1-cm defect in the left sciatic nerve and ENT, 32 adults female Wistar albino rats were separated into 4 groups (n = 8 for each) including ENT per se (group 1; ENT group) and ENT plus intratubal ASC injection groups killed on day 21 (group 2; ENT-ASC-21-day group), 60 days (group 3; ENT-ASC-60-day group), and 120 days (group 4; ENT-ASC-120-day group). Functional (sciatic function index, hip circumference, withdrawal reflex latency, muscle weight ratio), electrophysiological, histomorphometric, and immunohistochemical analyses were performed in each group. Sciatic function index was significantly higher (?51.98 ± 5.94, P < 0.01) and withdrawal reflex latency was shorter (?6.21 ± 2.14, P < 0.01), in the group 4 as compared with all other groups on day 21. Amplitude of contraction was significantly lower in the group 4 as compared with all other groups (0.22 ± 0.05 vs 0.34 ± 0.07, 0.50 ± 0.11, and 0.61 ± 0.16, P < 0.01 for each). Immunohistochemical analysis revealed presence of green fluorescent protein, vimentin-stained cells, and single neural progenitor cells indicating that induction of neuronal differentiation by ASCs and direct involvement of ASCs within the axonal structure alongside extension of ASCs to the muscular layer of the group 4. In conclusion, our findings revealed that use of ENT plus intratubal ASC injection in a rat sciatic nerve transection model was associated with satisfactory functional outcome and improved peripheral axonal regeneration along with stem cell neural differentiation.

Açıklama

Anahtar Kelimeler

Adipose Tissue–derived Stem Cells, Epineural Tubulization, Peripheral Nerve Injury, Rat Model, Sciatic Nerve Transection

Kaynak

Annals of Plastic Surgery

WoS Q Değeri

Q3

Scopus Q Değeri

N/A

Cilt

Sayı

Künye

Karakol, P., Kapı, E., Karaöz, E., Tunik, S., & Bozkurt, M. (2021). Comparison of the Effects of Intratubal Injection of Adipose-Derived Mesenchymal Stem Cells in a Rat Sciatic Nerve Transection: An Experimental Study. Annals of plastic surgery, 10.1097/SAP.0000000000003040. Advance online publication.