Comparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection: an experimental study

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorKarakol, Perçin
dc.contributor.authorKapı, Emin
dc.contributor.authorKaraöz, Erdal
dc.contributor.authorTunik, Selçuk
dc.contributor.authorBozkurt, Mehmet
dc.date.accessioned2021-11-10T13:33:41Z
dc.date.available2021-11-10T13:33:41Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractThis study was designed to evaluate the efficacy of epineural tubulization (ENT) with or without intratubal application of adipose-derived mesenchymal stem cells (ASCs) in the rat model of sciatic nerve transection. After formation of 1-cm defect in the left sciatic nerve and ENT, 32 adults female Wistar albino rats were separated into 4 groups (n = 8 for each) including ENT per se (group 1; ENT group) and ENT plus intratubal ASC injection groups killed on day 21 (group 2; ENT-ASC-21-day group), 60 days (group 3; ENT-ASC-60-day group), and 120 days (group 4; ENT-ASC-120-day group). Functional (sciatic function index, hip circumference, withdrawal reflex latency, muscle weight ratio), electrophysiological, histomorphometric, and immunohistochemical analyses were performed in each group. Sciatic function index was significantly higher (?51.98 ± 5.94, P < 0.01) and withdrawal reflex latency was shorter (?6.21 ± 2.14, P < 0.01), in the group 4 as compared with all other groups on day 21. Amplitude of contraction was significantly lower in the group 4 as compared with all other groups (0.22 ± 0.05 vs 0.34 ± 0.07, 0.50 ± 0.11, and 0.61 ± 0.16, P < 0.01 for each). Immunohistochemical analysis revealed presence of green fluorescent protein, vimentin-stained cells, and single neural progenitor cells indicating that induction of neuronal differentiation by ASCs and direct involvement of ASCs within the axonal structure alongside extension of ASCs to the muscular layer of the group 4. In conclusion, our findings revealed that use of ENT plus intratubal ASC injection in a rat sciatic nerve transection model was associated with satisfactory functional outcome and improved peripheral axonal regeneration along with stem cell neural differentiation.en_US
dc.identifier.citationKarakol, P., Kapı, E., Karaöz, E., Tunik, S., & Bozkurt, M. (2021). Comparison of the Effects of Intratubal Injection of Adipose-Derived Mesenchymal Stem Cells in a Rat Sciatic Nerve Transection: An Experimental Study. Annals of plastic surgery, 10.1097/SAP.0000000000003040. Advance online publication.en_US
dc.identifier.doi10.1097/SAP.0000000000003040en_US
dc.identifier.pmid34711729en_US
dc.identifier.scopus2-s2.0-85127730042en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1097/SAP.0000000000003040
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2216
dc.identifier.wosWOS:000771079500022en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdal
dc.language.isoenen_US
dc.publisherWolters Kluweren_US
dc.relation.ispartofAnnals of Plastic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipose Tissue–derived Stem Cellsen_US
dc.subjectEpineural Tubulizationen_US
dc.subjectPeripheral Nerve Injuryen_US
dc.subjectRat Modelen_US
dc.subjectSciatic Nerve Transectionen_US
dc.titleComparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection: an experimental studyen_US
dc.typeArticleen_US

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