Acousto-holographic reconstruction of whole-cell stiffness maps

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dc.authoridGülsüm Gençoğlan / 0000-0002-0650-0722en_US
dc.authorscopusidGülsüm Gençoğlan / 56074135100en_US
dc.authorwosidGülsüm Gençoğlan / CQE-8195-2022en_US
dc.contributor.authorVarol, Rahmetullah
dc.contributor.authorKaravelioğlu, Zeynep
dc.contributor.authorÖmeroğlu, Sevde
dc.contributor.authorAydemir, Gizem
dc.contributor.authorKaradağ, Aslıhan
dc.contributor.authorMeco, Hanife E.
dc.contributor.authorDemirçalı, Ali A.
dc.contributor.authorYılmaz, Abdurrahim
dc.contributor.authorKoçal, Gizem C.
dc.contributor.authorGençoğlan, Gülsüm
dc.contributor.authorOruç, Muhammed E.
dc.contributor.authorEsmer, Gökhan B.
dc.contributor.authorBaşbınar, Yasemin
dc.contributor.authorÖzdemir, Şahin K.
dc.contributor.authorÜvet, Hüseyin
dc.date.accessioned2022-12-12T05:35:51Z
dc.date.available2022-12-12T05:35:51Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractAccurate assessment of cell stiffness distribution is essential due to the critical role of cell mechanobiology in regulation of vital cellular processes like proliferation, adhesion, migration, and motility. Stiffness provides critical information in understanding onset and progress of various diseases, including metastasis and differentiation of cancer. Atomic force microscopy and optical trapping set the gold standard in stiffness measurements. However, their widespread use has been hampered with long processing times, unreliable contact point determination, physical damage to cells, and unsuitability for multiple cell analysis. Here, we demonstrate a simple, fast, label-free, and high-resolution technique using acoustic stimulation and holographic imaging to reconstruct stiffness maps of single cells. We used this acousto-holographic method to determine stiffness maps of HCT116 and CTC-mimicking HCT116 cells and differentiate between them. Our system would enable widespread use of whole-cell stiffness measurements in clinical and research settings for cancer studies, disease modeling, drug testing, and diagnostics. © 2022, The Author(s).en_US
dc.identifier.citationVarol, R., Karavelioglu, Z., Omeroglu, S., Aydemir, G., Karadag, A., Meco, H. E., ... & Uvet, H. (2022). Acousto-holographic reconstruction of whole-cell stiffness maps. Nature Communications, 13(1), 1-10.en_US
dc.identifier.doi10.1038/s41467-022-35075-xen_US
dc.identifier.isbn20411723
dc.identifier.issue1en_US
dc.identifier.pmid36446776en_US
dc.identifier.scopus2-s2.0-85142922318en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttp://dx.doi.org/10.1038/s41467-022-35075-x
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3460
dc.identifier.volume13en_US
dc.identifier.wosWOS:000890398000007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorGençoğlan, Gülsüm
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.relation.ispartofNature Communicationsen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcoustic Stimulationen_US
dc.subjectBiophysicsen_US
dc.subjectCell Differentiationen_US
dc.subjectHolographyen_US
dc.subjectOptical Tweezersen_US
dc.titleAcousto-holographic reconstruction of whole-cell stiffness mapsen_US
dc.typeArticleen_US

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