Neuronal regeneration in injured rat spinal cord after human dental pulp derived neural crest stem cell transplantation

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Küçük Resim

Tarih

2018

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Comenius Univ

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

OBJECTIVE: This study aimed to analyze the effect of human Dental Pulp-Neural Crest Stem Cells (hDP-NCSCs) delivery on lesion site after spinal cord injury (SCI), and to observe the functional recovery after transplantation. METHODS: Neural Crest Stem Cells (NCSCs) were isolated from human Dental Pulp (hDP). The experimental rat population was divided into four groups (n = 6/24). Their behavioral motility was scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords were examined for Green Fluorescent Protein (GFP) labeled hDP-NCSCs by immunofluorescence (IF) staining. RESULTS: In early post-injury (p.i) period, the ultrastructure of spinal cord tissue was preserved in Group 4. The majority of cells forming the ependymal region around the central canal were found to be hDP-NCSCs. While the grey-and-white-matter around the ependymal region was composed of e.g. GFP cells, with astrocytic-like appearance. The scores showed significant motor recovery in hind limb functions in Group 4. However, no obvious change was observed in other groups. C ONCLUSION: Cells e.g., mesenchymal (Vimentin+) which express GFP+ cells in the gray-and-white-matter around the ependymal region could indicate the potential to self-renewal and plasticity. Thus, transplantation of hDP-NCSCs might be an effective strategy to improve functional recovery following spinal cord trauma (Fig. 10, Ref. 32). Text in PDF www.elis.sk.

Açıklama

Anahtar Kelimeler

Functional Recovery, Human Dental Pulp, Neural Crest Stem Cells, Spinal Cord Injury

Kaynak

Bratislava Medical Journal-Bratislavske Lekarske Listy

WoS Q Değeri

Q3

Scopus Q Değeri

Q3

Cilt

119

Sayı

3

Künye

Kabatas, S., Demir, C. S., Civelek, E., Yilmaz, I., Kircelli, A., Yilmaz, C., … Karaoz, E. (2018). Neuronal regeneration in injured rat spinal cord after human dental pulp derived neural crest stem cell transplantation. Bratislava Medical Journal, 119(3), 143–151. https://doi.org/10.4149/BLL_2018_028