Kruppel-Like transcription factor-4 gene expression and DNA methylation status in type 2 diabetes and diabetic nephropathy patients

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Elsevier Science Inc

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Background/Aim. Diabetic nephropathy (DN) is one of the most serious microvascular complications in diabetic patients. The kruppel-like transcription factor-4 (KLF-4) affects the expression of genes involved in the pathogenesis of DN. The present study aims to identify the KLF-4 expression and DNA methylation (DNAMe) status in patients with type-2 diabetes (T2D) and DN and to reveal the contribution of the KLF-4 to the development of DN. Material and Methods. The cohort study was performed with blood samples from 120 individuals; T2D group (n = 40), DN group (n = 40) and control group (n = 40). The expression level of the KLF-4 gene was analyzed using the real-time polymerase chain reaction (qRT-PCR) and the methylation profile detected using the methylation-specific PCR (MS-PCR) technique. Results. According to our findings, KLF-4 mRNA expression in the T2D group was 1.60 fold lower than in the control group (p = 0.001). In the DN group, the expression of KLF-4 mRNA was 2.92-fold less than that of the T2D group (p = 0.001). There was no significant alteration in the DNAMe status among the groups. Conclusion. Our findings showed that regardless of the DNAMe status, KLF-4 gene expression may play a role in the development of T2D and DN. This suggests that the KLF-4 gene may be the target gene in understanding the mechanism of nephropathy, which is the most important complication of diabetes, and planning nephropathy-related treatments, but the data should be supported with more studies. (C) 2019 IMSS. Published by Elsevier Inc.


Anahtar Kelimeler

Diabetic Nephropathy, Dna Methylation, Epigenetics, Kruppel-Like Transcription Factor-4, Type 2 Diabetes


Archives of Medical Research

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Coskun, Z. M., Ersoz, M., Adas, M., Hancer, V. S., Boysan, S. N., Gonen, M. S., & Acar, A. (2019). Kruppel-Like Transcription Factor-4 Gene Expression and DNA Methylation Status in Type 2 Diabetes and Diabetic Nephropathy Patients. Archives of medical research, 50(3), 91-97.