Kruppel-Like transcription factor-4 gene expression and DNA methylation status in type 2 diabetes and diabetic nephropathy patients

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dc.authoridVeysel Sabri Hançer / 0000-0003-2994-1077en_US
dc.authorscopusidVeysel Sabri Hançer / 6506533543
dc.authorwosidVeysel Sabri Hançer / X-8971-2018
dc.contributor.authorCoşkun, Zeynep Mine
dc.contributor.authorErsöz, Melike
dc.contributor.authorAdaş, Mine
dc.contributor.authorHançer, Veysel Sabri
dc.contributor.authorBoysan, Şerife Nur
dc.contributor.authorGönen, Mustafa Sait
dc.contributor.authorAcar, Aynur
dc.date.accessioned2020-08-30T20:06:51Z
dc.date.available2020-08-30T20:06:51Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground/Aim. Diabetic nephropathy (DN) is one of the most serious microvascular complications in diabetic patients. The kruppel-like transcription factor-4 (KLF-4) affects the expression of genes involved in the pathogenesis of DN. The present study aims to identify the KLF-4 expression and DNA methylation (DNAMe) status in patients with type-2 diabetes (T2D) and DN and to reveal the contribution of the KLF-4 to the development of DN. Material and Methods. The cohort study was performed with blood samples from 120 individuals; T2D group (n = 40), DN group (n = 40) and control group (n = 40). The expression level of the KLF-4 gene was analyzed using the real-time polymerase chain reaction (qRT-PCR) and the methylation profile detected using the methylation-specific PCR (MS-PCR) technique. Results. According to our findings, KLF-4 mRNA expression in the T2D group was 1.60 fold lower than in the control group (p = 0.001). In the DN group, the expression of KLF-4 mRNA was 2.92-fold less than that of the T2D group (p = 0.001). There was no significant alteration in the DNAMe status among the groups. Conclusion. Our findings showed that regardless of the DNAMe status, KLF-4 gene expression may play a role in the development of T2D and DN. This suggests that the KLF-4 gene may be the target gene in understanding the mechanism of nephropathy, which is the most important complication of diabetes, and planning nephropathy-related treatments, but the data should be supported with more studies. (C) 2019 IMSS. Published by Elsevier Inc.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul Bilim University [2015017]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Coordination Unit of Istanbul Bilim University. Project no: 2015017.en_US
dc.identifier.citationCoskun, Z. M., Ersoz, M., Adas, M., Hancer, V. S., Boysan, S. N., Gonen, M. S., & Acar, A. (2019). Kruppel-Like Transcription Factor-4 Gene Expression and DNA Methylation Status in Type 2 Diabetes and Diabetic Nephropathy Patients. Archives of medical research, 50(3), 91-97.en_US
dc.identifier.doi10.1016/j.arcmed.2019.05.012en_US
dc.identifier.endpage97en_US
dc.identifier.issn0188-4409en_US
dc.identifier.issn1873-5487en_US
dc.identifier.issue3en_US
dc.identifier.pmid31495395en_US
dc.identifier.scopus2-s2.0-85069661283en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://doi.org/10.1016/j.arcmed.2019.05.012
dc.identifier.urihttps://hdl.handle.net/20.500.12713/636
dc.identifier.volume50en_US
dc.identifier.wosWOS:000487168200003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHançer, Veysel Sabrien_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofArchives of Medical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDiabetic Nephropathyen_US
dc.subjectDna Methylationen_US
dc.subjectEpigeneticsen_US
dc.subjectKruppel-Like Transcription Factor-4en_US
dc.subjectType 2 Diabetesen_US
dc.titleKruppel-Like transcription factor-4 gene expression and DNA methylation status in type 2 diabetes and diabetic nephropathy patientsen_US
dc.typeArticleen_US

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