Optimization of methacrylated gelatin /layered double hydroxides nanocomposite cell-laden hydrogel bioinks with high printability for 3D extrusion bioprinting

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dc.authoridAyça Bal Öztürk / 0000-0002-6502-528Xen_US
dc.authoridBurçin İzbudak / 0000-0002-5572-7966
dc.authorscopusidAyça Bal Öztürk / 57062000100en_US
dc.authorscopusidBurçin İzbudak / 57221779519
dc.authorwosidAyça Bal Öztürk / M-4472-2018en_US
dc.authorwosidBurçin İzbudak / GXP-7807-2022
dc.contributor.authorAlarçin, Emine
dc.contributor.authorİzbudak, Burçin
dc.contributor.authorYüce Erarslan, Elif
dc.contributor.authorDomingo, Sherif
dc.contributor.authorTutar, Rümeysa
dc.contributor.authorTiti, Kariman
dc.contributor.authorKocaağa, Banu
dc.contributor.authorGüner, F. Seniha
dc.contributor.authorBal Öztürk, Ayça
dc.date.accessioned2022-11-08T13:48:37Z
dc.date.available2022-11-08T13:48:37Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Temel Bilimleri Bölümüen_US
dc.description.abstractLayered double hydroxides (LDHs) offer unique source of inspiration for design of bone mimetic biomaterials due to their superior mechanical properties, drug delivery capability and regulation cellular behaviors, particularly by divalent metal cations in their structure. Three-dimensional (3D) bioprinting of LDHs holds great promise as a novel strategy thanks to highly tunable physiochemical properties and shear-thinning ability of LDHs, which allow shape fidelity after deposition. Herein, we introduce a straightforward strategy for extrusion bioprinting of cell laden nanocomposite hydrogel bioink of gelatin methacryloyl (GelMA) biopolymer and LDHs nanoparticles. First, we synthesized LDHs by co-precipitation process and systematically examined the effect of LDHs addition on printing parameters such as printing pressure, extrusion rate, printing speed, and finally bioink printability in creating grid-like constructs. The developed hydrogel bioinks provided precise control over extrudability, extrusion uniformity, and structural integrity after deposition. Based on the printability and rheological analysis, the printability could be altered by controlling the concentration of LDHs, and printability was found to be ideal with the addition of 3 wt % LDHs. The addition of LDHs resulted in remarkably enhanced compressive strength from 652 kPa (G-LDH0) to 1168 kPa (G-LDH3). It was shown that the printed nanocomposite hydrogel scaffolds were able to support encapsulated osteoblast survival, spreading, and proliferation in the absence of any osteoinductive factors taking advantage of LDHs. In addition, cells encapsulated in G-LDH3 had a larger cell spreading area and higher cell aspect ratio than those encapsulated in G-LDH0. Altogether, the results demonstrated that the developed GelMA/LDHs nanocomposite hydrogel bioink revealed a high potential for extrusion bioprinting with high structural fidelity to fabricate implantable 3D hydrogel constructs for repair of bone defects.en_US
dc.identifier.citationAlarçin, E., İzbudak, B., Yüce Erarslan, E., Domingo, S., Tutar, R., Titi, K., . . . Bal-Öztürk, A. (2022). Optimization of methacrylated gelatin /layered double hydroxides nanocomposite cell-laden hydrogel bioinks with high printability for 3D extrusion bioprinting. Journal of Biomedical Materials Research - Part A, doi:10.1002/jbm.a.37450en_US
dc.identifier.doi10.1002/jbm.a.37450en_US
dc.identifier.scopus2-s2.0-85139487552en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1002/jbm.a.37450
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3286
dc.identifier.wosWOS:000865405500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBal Öztürk, Ayça
dc.institutionauthorİzbudak, Burçin
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Incen_US
dc.relation.ispartofJournal of Biomedical Materials Research - Part Aen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3D Bioprintingen_US
dc.subjectHydrogel Bioinken_US
dc.subjectLayered Double Hydroxideen_US
dc.subjectNanocomposite Scaffolden_US
dc.titleOptimization of methacrylated gelatin /layered double hydroxides nanocomposite cell-laden hydrogel bioinks with high printability for 3D extrusion bioprintingen_US
dc.typeArticleen_US

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