Formulating and characterizing an exosome-based dopamine carrier system
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Jove
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Exosomes between 40 and 200 nm in size constitute the smallest subgroup of extracellular vesicles. These bioactive vesicles secreted by cells play an active role in intercellular cargo and communication. Exosomes are mostly found in body fluids such as plasma, cerebrospinal fluid, urine, saliva, amniotic fluid, colostrum, breast milk, joint fluid, semen, and pleural acid. Considering the size of exosomes, it is thought that they may play an important role in central nervous system diseases because they can pass through the blood-brain barrier (BBB). Hence, this study aimed to develop an exosome-based nanocarrier system by encapsulating dopamine into exosomes isolated from Wharton's jelly mesenchymal stem cells (WJ-MSCs). Exosomes that passed the characterization process were incubated with dopamine. The dopamine-loaded exosomes were recharacterized at the end of incubation. Dopamine-loaded exosomes were investigated in drug release and cytotoxicity assays. The results showed that dopamine could be successfully encapsulated within the exosomes and that the dopamine-loaded exosomes did not affect fibroblast viability.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of Visualized Experiment
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
182
Sayı
Künye
Yavuz B, Darici H, Zorba Yildiz AP, Abamor EŞ, Topuzoğullari M, Bağirova M, Allahverdiyev A, Karaoz E. Formulating and Characterizing an Exosome-based Dopamine Carrier System. J Vis Exp. 2022 Apr 4;(182). doi: 10.3791/63624. PMID: 35435916.
