Formulating and characterizing an exosome-based dopamine carrier system

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dc.authoridMüslüm Şahin / 0000-0003-4976-6720en_US
dc.authoridTolga Sinan Güvenç / 0000-0002-6738-266Xen_US
dc.authorscopusidTolga Sinan Güvenç / 6602421975
dc.authorscopusidMüslüm Şahin / 41862126900
dc.authorwosidMüslüm Şahin / DNT-9358-2022en_US
dc.authorwosidTolga Sinan Güvenç / JQN-5460-2023
dc.contributor.authorNaser, Abdulrahman
dc.contributor.authorIsgandarov, Khagani
dc.contributor.authorGüvenç, Tolga Sinan
dc.contributor.authorEkmekçi, Ahmet
dc.contributor.authorGüvenç, Rengin Çetin
dc.contributor.authorŞahin, Müslüm
dc.contributor.authorGündüz, Sabahattin
dc.contributor.authorŞahin, Müslüm
dc.date.accessioned2022-05-20T13:27:18Z
dc.date.available2022-05-20T13:27:18Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractExosomes between 40 and 200 nm in size constitute the smallest subgroup of extracellular vesicles. These bioactive vesicles secreted by cells play an active role in intercellular cargo and communication. Exosomes are mostly found in body fluids such as plasma, cerebrospinal fluid, urine, saliva, amniotic fluid, colostrum, breast milk, joint fluid, semen, and pleural acid. Considering the size of exosomes, it is thought that they may play an important role in central nervous system diseases because they can pass through the blood-brain barrier (BBB). Hence, this study aimed to develop an exosome-based nanocarrier system by encapsulating dopamine into exosomes isolated from Wharton's jelly mesenchymal stem cells (WJ-MSCs). Exosomes that passed the characterization process were incubated with dopamine. The dopamine-loaded exosomes were recharacterized at the end of incubation. Dopamine-loaded exosomes were investigated in drug release and cytotoxicity assays. The results showed that dopamine could be successfully encapsulated within the exosomes and that the dopamine-loaded exosomes did not affect fibroblast viability.en_US
dc.identifier.citationYavuz B, Darici H, Zorba Yildiz AP, Abamor EŞ, Topuzoğullari M, Bağirova M, Allahverdiyev A, Karaoz E. Formulating and Characterizing an Exosome-based Dopamine Carrier System. J Vis Exp. 2022 Apr 4;(182). doi: 10.3791/63624. PMID: 35435916.en_US
dc.identifier.doi10.3791/63624en_US
dc.identifier.issn2578-2614en_US
dc.identifier.pmid35435916en_US
dc.identifier.scopus2-s2.0-85128792693en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttp://doi.org/10.3791/63624
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2695
dc.identifier.volume182en_US
dc.identifier.wosWOS:000810726600012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞahin, Müslüm
dc.institutionauthorGüvenç, Tolga Sinan
dc.language.isoenen_US
dc.publisherJoveen_US
dc.relation.ispartofJournal of Visualized Experimenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleFormulating and characterizing an exosome-based dopamine carrier systemen_US
dc.typeArticleen_US

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