Hybrid extracellular vesicles-liposome incorporated advanced bioink to deliver microRNA
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
IOP Publishing
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In additive manufacturing, bioink formulations govern strategies to engineer 3D living tissues that mimic the complex architectures and functions of native tissues for successful tissue regeneration. Conventional 3D-printed tissues are limited in their ability to alter the fate of laden cells. Specifically, the efficient delivery of gene expression regulators (i.e. microRNAs (miRNAs)) to cells in bioprinted tissues has remained largely elusive. In this study, we explored the inclusion of extracellular vesicles (EVs), naturally occurring nanovesicles (NVs), into bioinks to resolve this challenge. EVs show excellent biocompatibility, rapid endocytosis, and low immunogenicity, which lead to the efficient delivery of miRNAs without measurable cytotoxicity. EVs were fused with liposomes to prolong and control their release by altering their physical interaction with the bioink. Hybrid EVs-liposome (hEL) NVs were embedded in gelatin-based hydrogels to create bioinks that could efficiently encapsulate and deliver miRNAs at the target site in a controlled and sustained manner. The regulation of cells' gene expression in a 3D bioprinted matrix was achieved using the hELs-laden bioink as a precursor for excellent shape fidelity and high cell viability constructs. Novel regulatory factors-loaded bioinks will expedite the translation of new bioprinting applications in the tissue engineering field.
Açıklama
Anahtar Kelimeler
3D Bioprinting, Bioink, Extracellular Vesicles, Liposomes, microRNAs, Tissue Regeneration
Kaynak
Biofabrication
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
14
Sayı
4
Künye
Elkhoury K, Chen M, Koçak P, Enciso-Martínez E, Bassous NJ, Lee MC, Byambaa B, Rezaei Z, Li Y, Ubina López ME, Gurian M, Sobahi N, Hussain MA, Sanchez-Gonzalez L, Leijten J, Hassan S, Arab-Tehrany E, Ward JE, Shin SR. Hybrid extracellular vesicles-liposome incorporated advanced bioink to deliver microRNA. Biofabrication. 2022 Aug 19;14(4). doi: 10.1088/1758-5090/ac8621. PMID: 35917808.
