Hybrid extracellular vesicles-liposome incorporated advanced bioink to deliver microRNA

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dc.authoridPolen Koçak / 0000-0003-4493-1061en_US
dc.authorscopusidPolen Koçak / 57191971267
dc.authorwosidPolen Koçak / JHP-3402-2023
dc.contributor.authorElkhoury, Kamil
dc.contributor.authorChen, Mo
dc.contributor.authorKoçak, Polen
dc.contributor.authorEnciso-Martínez, Eduardo
dc.contributor.authorBassous, Nicole Joy
dc.contributor.authorChul Lee, Myung
dc.contributor.authorByambaa, Batzaya
dc.contributor.authorRezaei, Zahra
dc.contributor.authorLi, Yang
dc.contributor.authorUbina López , María Elizabeth
dc.contributor.authorGurian, Melvin
dc.contributor.authorSobahi, Nebras
dc.contributor.authorAsif Hussain, Mohammad
dc.contributor.authorSanchez-Gonzalez, Laura
dc.contributor.authorLeijten, Jeroen
dc.contributor.authorHassan, Shabir
dc.contributor.authorArab-Tehrany, Elmira
dc.contributor.authorEllis Ward, Jennifer
dc.contributor.authorShin, Su Ryon
dc.date.accessioned2022-09-05T07:40:33Z
dc.date.available2022-09-05T07:40:33Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.description.abstractIn additive manufacturing, bioink formulations govern strategies to engineer 3D living tissues that mimic the complex architectures and functions of native tissues for successful tissue regeneration. Conventional 3D-printed tissues are limited in their ability to alter the fate of laden cells. Specifically, the efficient delivery of gene expression regulators (i.e. microRNAs (miRNAs)) to cells in bioprinted tissues has remained largely elusive. In this study, we explored the inclusion of extracellular vesicles (EVs), naturally occurring nanovesicles (NVs), into bioinks to resolve this challenge. EVs show excellent biocompatibility, rapid endocytosis, and low immunogenicity, which lead to the efficient delivery of miRNAs without measurable cytotoxicity. EVs were fused with liposomes to prolong and control their release by altering their physical interaction with the bioink. Hybrid EVs-liposome (hEL) NVs were embedded in gelatin-based hydrogels to create bioinks that could efficiently encapsulate and deliver miRNAs at the target site in a controlled and sustained manner. The regulation of cells' gene expression in a 3D bioprinted matrix was achieved using the hELs-laden bioink as a precursor for excellent shape fidelity and high cell viability constructs. Novel regulatory factors-loaded bioinks will expedite the translation of new bioprinting applications in the tissue engineering field.en_US
dc.identifier.citationElkhoury K, Chen M, Koçak P, Enciso-Martínez E, Bassous NJ, Lee MC, Byambaa B, Rezaei Z, Li Y, Ubina López ME, Gurian M, Sobahi N, Hussain MA, Sanchez-Gonzalez L, Leijten J, Hassan S, Arab-Tehrany E, Ward JE, Shin SR. Hybrid extracellular vesicles-liposome incorporated advanced bioink to deliver microRNA. Biofabrication. 2022 Aug 19;14(4). doi: 10.1088/1758-5090/ac8621. PMID: 35917808.en_US
dc.identifier.doi10.1088/1758-5090/ac8621en_US
dc.identifier.issn1758-5082en_US
dc.identifier.issue4en_US
dc.identifier.pmid35917808en_US
dc.identifier.scopus2-s2.0-85136541312en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttp://doi.org/10.1088/1758-5090/ac8621
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3119
dc.identifier.volume14en_US
dc.identifier.wosWOS:000842242600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKoçak, Polen
dc.language.isoenen_US
dc.publisherIOP Publishingen_US
dc.relation.ispartofBiofabricationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3D Bioprintingen_US
dc.subjectBioinken_US
dc.subjectExtracellular Vesiclesen_US
dc.subjectLiposomesen_US
dc.subjectmicroRNAsen_US
dc.subjectTissue Regenerationen_US
dc.titleHybrid extracellular vesicles-liposome incorporated advanced bioink to deliver microRNAen_US
dc.typeArticleen_US

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