Comparison of mesenchymal stem cell sheets and chondrocyte sheets in a rabbit growth plate injury model
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background/aim: The treatment of posttraumatic deformities and differences in length between the extremities resulting from physeal injury remains controversial. The aims of this study were to compare the efficacy of tissue-engineered, monolayer, and allogeneic mesenchymal stem cell sheets and chondrocyte sheets for physeal arrest treatment and to investigate cell sheet technology as a novel method for cell transplantation in physeal cartilage repair. Materials and methods: A proximal tibial physeal injury was induced in New Zealand rabbits. Allogeneic mesenchymal stem cells (MSCs) and chondrocytes were cultured in temperature-responsive culture dishes and applied to the iatrogenic partial growth plate defects in single-sheet grafts (cell sheets). Treatment efficacy was determined using radiological measurements, as well as histological and immunohistochemical staining. Results: Treatment with MSCs and chondrocytes prevented endochondral ossification in the physeal plate, and bone growth resumed after treatment in both the MSC and chondrocyte cell groups. We found significant differences in radiological evaluations between pre-and posttreatment measurements in both MSC and chondrocyte groups. Transplanted cells were observed in the damaged area in both of the groups, which differentiated in the direction of growth plate cartilage. Conclusion: Our results support the hypothesis that MSC or chondrocyte transplantation using the cell-sheet technique described in the present study aids in the regeneration of cartilage tissue during physeal arrest after growth plate damage.
Açıklama
Anahtar Kelimeler
Mesenchymal Stem Cell, Chondrocytes, Cell Sheet, Physeal Arrest
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
50
Sayı
4
Künye
Gültekin, A., Ağirdil, Y., Duman, B. Ö., DEMİR, C. S., & Karaöz, E. (2020). Comparison of mesenchymal stem cell sheets and chondrocyte sheets in a rabbit growth plate injury model. Turkish journal of medical sciences, 50(4), 1082.
