From experiments to simulation: Shear-induced responses of red blood cells to different oxygen saturation levels

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dc.authoridŞenol Pişkin / 0000-0002-8799-9472en_US
dc.authorscopusidŞenol Pişkin / 8636421200
dc.authorwosidŞenol Pişkin / JYJ-0063-2024
dc.contributor.authorUgurel, Elif
dc.contributor.authorPişkin, Şenol
dc.contributor.authorAksu, Ali Cenk
dc.contributor.authorEser, Aysenur
dc.contributor.authorYalcin, Ozlem
dc.date.accessioned2020-08-30T20:06:17Z
dc.date.available2020-08-30T20:06:17Z
dc.date.issued2020
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Makine Mühendisliği Bölümüen_US
dc.descriptionEser, Aysenur/0000-0002-9010-238X; Piskin, Senol/0000-0002-8799-9472en_US
dc.description.abstractRed blood cells (RBC) carry and deliver oxygen (O-2) to peripheral tissues through different microcirculatory regions where they are exposed to various levels of shear stress (SS). O-2 affinity of hemoglobin (Hb) decreases as the blood enters the microcirculation. This phenomenon determines Hb interactions with RBC membrane proteins that can further regulate the structure of cytoskeleton and affect the mechanical properties of cells. The goal of this study is to evaluate shear-induced RBC deformability and simulate RBC dynamics in blood flow under oxygenated and deoxygenated conditions. Venous blood samples from healthy donors were oxygenated with ambient air or deoxygenated with 100% nitrogen gas for 10 min and immediately applied into an ektacytometer (LORRCA). RBC deformability was measured before and after the application of continuous 5 Pa SS for 300 s by LORRCA and recorded as elongation index (EI) values. A computational model was generated for the simulation of blood flow in a real carotid artery section. EI distribution throughout the artery and its relationships with velocity, pressure, wall SS and viscosity were determined by computational tools. RBC deformability significantly increased in deoxygenation compared to oxygenated state both before and after 5 Pa SS implementation (p < 0.0001). However, EI values after continuous SS were not significant at higher SS levels (>5.15 Pa) in deoxygenated condition. Simulation results revealed that the velocity gradient dominates the generation of SS and the shear thinning effect of blood has a minor effect on it. Distribution of EI was calculated during oxygenation/deoxygenation which is 5-10 times higher around the vessel wall compared to the center of the lumen for sections of the pulsatile flow profile. The extent of RBC deformability increases as RBCs approach to the vessel wall in a real 3D artery model and this increment is higher for deoxygenated condition compared to the oxygenated state. Hypoxia significantly increases shear-induced RBC deformability. RBCs could regulate their own mechanical properties in blood flow by increasing their deformability in hypoxic conditions. Computational tools can be applied for defining hypoxia-mediated RBC deformability changes to monitor blood flow in hypoxic tissues.en_US
dc.description.sponsorshipTurkish Scientific and Technical CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-214S186]en_US
dc.description.sponsorshipThis study was supported by the Turkish Scientific and Technical Council grant SBAG-214S186.en_US
dc.identifier.citationUgurel, E., Piskin, S., Aksu, A. C., Eser, A., & Yalcin, O. (2020). From Experiments to Simulation: Shear-Induced Responses of Red Blood Cells to Different Oxygen Saturation Levels. FRONTIERS IN PHYSIOLOGY, 10. https://doi.org/10.3389/fphys.2019.01559en_US
dc.identifier.doi10.3389/fphys.2019.01559en_US
dc.identifier.issn1664-042Xen_US
dc.identifier.pmid32038272en_US
dc.identifier.scopus2-s2.0-85079073025en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3389/fphys.2019.01559
dc.identifier.urihttps://hdl.handle.net/20.500.12713/460
dc.identifier.volume10en_US
dc.identifier.wosWOS:000511329100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorPişkin, Şenolen_US
dc.language.isoenen_US
dc.publisherFrontiers Media Saen_US
dc.relation.ispartofFrontiers In Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRed Blood Cellen_US
dc.subjectDeformabilityen_US
dc.subjectOxygenationen_US
dc.subjectHypoxiaen_US
dc.subjectComputational Fluid Dynamicsen_US
dc.subjectPulsatile Blood Flowen_US
dc.titleFrom experiments to simulation: Shear-induced responses of red blood cells to different oxygen saturation levelsen_US
dc.typeArticleen_US

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