The investigation of the effects of postnatal alcohol exposure on molecular content and antioxidant capacity of mice liver tissue
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
One of the most common causes of fetal alcohol spectrum disease (FASD) characterized with neuro-developmental disorder and growth retardation, is the postnatal alcohol consumption. Since studies in literature are mainly focused on alcohol-induced effects on brain tissues, the molecular effects of postnatal alcohol con-sumption on fetal liver are not clarified yet. The aim of this study is to determine the postnatal alcohol consumption-induced structural and compositional changes on liver tissue and the antioxidant capacity of liver. Newborn mice were divided into 3 groups as control group without any treatment, alcohol group treated with 3.0 g/kg of ethanol in 0.02 ml/g of artificially enriched milk between Postnatal Days (PD) 3-20 and intragastric intubation control group which was intragastrically intubated in the same method as the alcohol group but without ethanol/milk. These postnatal days in mice refers prenatal period (third trimester) of gestation in human. The biomolecular changes were determined by ATR-FTIR spectral analysis of the samples, besides the biochemical measurement of total protein content and antioxidant capacity of liver tissue. The result of the current study shows that while there was a slight increase in total lipid content, significant decrease in unsat-urated lipid and total protein contents and total antioxidant capacity of liver were observed in alcohol-treated group. Thus, it is concluded that postnatal alcohol treatment causes significant changes in tissue proteins and lipids by inducing lipid peroxidation and changes in protein conformations of the liver tissue. In addition to that alcohol consumption also reduce the antioxidant capacity of liver tissue.
Description
Keywords
Postnatal Alcohol Consumption, Intragastric İntubation, ATR-FTIR Spectroscopy, Total Antioxidant Capacity, Mice, Liver
Journal or Series
LIFE SCIENCES
WoS Q Value
Q1
Scopus Q Value
Q1
Volume
310
Issue
Citation
Algburi, A. F., Dursun, I., & Ustaoglu, S. G. (2022). The investigation of the effects of postnatal alcohol exposure on molecular content and antioxidant capacity of mice liver tissue. Life Sciences, 310, 121102.
