Investigation of the efficacy of epidermal growth factor, boric acid and their combination in cartilage injury in rats: An experimental study

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dc.authoridCetin, Yuksel/0000-0001-5101-3870
dc.authoridInce, Sinan/0000-0002-1915-9797
dc.authoridYilmaz, Bilge Kagan/0000-0002-2765-7833
dc.authoridKONYA, MEHMET NURI/0000-0002-5877-8347
dc.authoridGungor, Aysen/0000-0003-4955-8059
dc.authorwosidCetin, Yuksel/AAS-7528-2020
dc.authorwosidInce, Sinan/I-2358-2019
dc.contributor.authorYilmaz, Bilge Kagan
dc.contributor.authorKonya, Mehmet Nuri
dc.contributor.authorInce, Sinan
dc.contributor.authorDemirel, Hasan Huseyin
dc.contributor.authorCetin, Yuksel
dc.contributor.authorGungor, Aysen
dc.date.accessioned2024-05-19T14:39:33Z
dc.date.available2024-05-19T14:39:33Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractObjectives: In this study, we aimed to determine the bioefficacy of epidermal growth factor (EGF), boric acid (BA), and their combination on cartilage injury in rats.Materials and methods: In in vitro setting, the cytotoxic effects of BA, EGF, and their combinations using mouse fibroblast cell (L929), human bone osteosarcoma cell (Saos-2), and human adipose derived mesenchymal stem cells (hAD-MSCs) were determined by applying MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] test. In in vivo setting, 72 rats were randomly divided into four groups. A standard chondral defect was created and microfracture was performed in all groups. Group A was determined as the control group. In addition to the standard procedure, Group B received 100 ng/mL of EGF, Group C received a combination of 100 ng/mL of EGF and 10 mu g/mL of BA combination, and Group D 20 mu g/mL of BA.Results: The cytotoxic effect of the combinations of EGF dilutions (1, 5, 10, 25, 50, 100, 200 ng/mL) with BA (100, 300, 500 mu g/mL) was observed only in the 72-h application period and in Saos-2. The cytotoxic effect of BA was reduced when combined with EGF. There was no significant difference in the histopathological scores among the groups (p=0.13). Conclusion: Our study showed that EGF and low-dose BA application had a positive effect on cartilage healing in rats. Significant decreases in recovery scores were observed in the other groups. The combination of EGF and BA promoted osteoblast growth. Detection of lytic lesions in the group treated with 20 mu g/mL of BA indicates that BA may have a cytotoxic effect.en_US
dc.identifier.doi10.52312/jdrs.2023.1074
dc.identifier.endpage168en_US
dc.identifier.issn2687-4792
dc.identifier.issue1en_US
dc.identifier.pmid38108177en_US
dc.identifier.scopus2-s2.0-85180272210en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage156en_US
dc.identifier.trdizinid1227237en_US
dc.identifier.urihttps://doi.org10.52312/jdrs.2023.1074
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1227237
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4802
dc.identifier.volume35en_US
dc.identifier.wosWOS:001114360100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTurkish Joint Diseases Foundationen_US
dc.relation.ispartofJoint Diseases and Related Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBoric Aciden_US
dc.subjectCartilage Regenerationen_US
dc.subjectDose Dependenten_US
dc.subjectEpidermal Growth Factoren_US
dc.subjectIn Vitroen_US
dc.subjectIn Vivoen_US
dc.titleInvestigation of the efficacy of epidermal growth factor, boric acid and their combination in cartilage injury in rats: An experimental studyen_US
dc.typeArticleen_US

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