Effect of potassium aluminum sulfate application on the viability of fibroblasts on a cad-cam feldspathic ceramic before and after thermocycling

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dc.authoridMustafa Borga Dönmez / 0000-0002-3094-7487en_US
dc.authorscopusidMustafa Borga Dönmez / 57202022054
dc.authorwosidMustafa Borga Dönmez / AGY-6155-2022en_US
dc.contributor.authorÇakmak, Gülce
dc.contributor.authorAkay, Canan
dc.contributor.authorDönmez, Mustafa Borga
dc.contributor.authorMumcu, Emre
dc.contributor.authorAkan, Handan Sevim
dc.contributor.authorSasany, Rafat
dc.contributor.authorAbou-Ayash, Samir
dc.contributor.authorYılmaz, Burak
dc.date.accessioned2022-06-28T07:50:18Z
dc.date.available2022-06-28T07:50:18Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractAbstract: Potassium aluminum sulfate (alum) is a known adjuvant, which has been used as a mordant in textile industry for color fixation. This material has potential to be incorporated into dentistry for color stability, yet its toxicity first needs to be evaluated. The present study aimed to evaluate the cytotoxic potential of potassium aluminum sulfate (alum) on fibroblasts when applied onto feldspathic ceramic before and after thermocycling. Forty-eight feldspathic ceramic specimens were divided into four groups (FC: no alum application or thermocycling; FCT: thermocycling without alum application; FA: alum application without thermocycling; FAT: alum application and thermocycling) (n = 12). Cell viability was assessed by using a tetrazolium salt 3-[4,5-dimethylthiazol2-yl]-2,5-diphnyltetrazolium bromide assay at 24 and 72 h, and cell cultures without any ceramic specimens served as control (C). One sample from each material group was further analyzed with energy dispersive X-ray spectroscopy (EDX). Cell viability at different time intervals within each group was analyzed with Friedman tests, while Kruskal–Wallis tests were used to compare the test groups within each time interval. Pairwise comparisons were further resolved by using Wilcoxon tests (a = 0.05). C had lower (p = 0.01) and FA had higher (p = 0.019) cell viability after 72 h. After 24 h, the highest cell viability was observed in C (p ? 0.036). After 72 h, the differences between C and FA, C and FAT, FC and FA, and FCT and FAT were nonsignificant (p > 0.05). Cell viability was not affected by alum application or thermocycling at any time interval (p ? 0.631). EDX analysis showed an increase in potassium concentration in FA and FAT when compared with FC and FCT. Regardless of the time interval, alum application onto feldspathic ceramic and thermocycling did not influence the cell viability.en_US
dc.identifier.citationÇakmak G, Akay C, Donmez MB, Mumcu E, Akan HS, Sasany R, Abou-Ayash S, Yilmaz B. Effect of Potassium Aluminum Sulfate Application on the Viability of Fibroblasts on a CAD-CAM Feldspathic Ceramic before and after Thermocycling. Materials (Basel). 2022 Jun 15;15(12):4232. doi: 10.3390/ma15124232. PMID: 35744291; PMCID: PMC9228813.en_US
dc.identifier.doi10.3390/ma15124232en_US
dc.identifier.issn1996-1944en_US
dc.identifier.issue12en_US
dc.identifier.pmid9228813en_US
dc.identifier.scopus2-s2.0-85132725594en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttp://doi.org/10.3390/ma15124232
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2952
dc.identifier.volume15en_US
dc.identifier.wosWOS:000816231500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDönmez, Mustafa Borga
dc.language.isoenen_US
dc.publisherPMIDen_US
dc.relation.ispartofMaterialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlumen_US
dc.subjectCytotoxicityen_US
dc.subjectFeldspathic Ceramicen_US
dc.subjectFibroblasten_US
dc.subjectThermocyclingen_US
dc.titleEffect of potassium aluminum sulfate application on the viability of fibroblasts on a cad-cam feldspathic ceramic before and after thermocyclingen_US
dc.typeArticleen_US

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