Editing SOX genes by CRISPR-Cas: current insights and future perspectives
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and its associated proteins (Cas) is an adaptive immune system in archaea and most bacteria. By repurposing these systems for use in eukaryote cells, a substantial revolution has arisen in the genome engineering field. In recent years, CRISPR-Cas technology was rapidly developed and different types of DNA or RNA sequence editors, gene activator or repressor, and epigenome modulators established. The versatility and feasibility of CRISPR-Cas technology has introduced this system as the most suitable tool for discovering and studying the mechanism of specific genes and also for generating appropriate cell and animal models. SOX genes play crucial roles in development processes and stemness. To elucidate the exact roles of SOX factors and their partners in tissue hemostasis and cell regener-ation, generating appropriate in vitro and in vivo models is crucial. In line with these premises, CRISPR-Cas technology is a promising tool for studying different family members of SOX transcription factors. In this review, we aim to highlight the importance of CRISPR-Cas and summarize the applications of this novel, promising technology in studying and decoding the function of different members of the SOX gene family. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland.
Açıklama
Anahtar Kelimeler
Cancer, CRISPR, Gene Editing, SOX Transcription Factors, Stem Cells
Kaynak
International Journal of Molecular Sciences
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
22
Sayı
21
Künye
Dehshahri A, Biagioni A, Bayat H, Lee EHC, Hashemabadi M, Fekri HS, Zarrabi A, Mohammadinejad R, Kumar AP. Editing SOX Genes by CRISPR-Cas: Current Insights and Future Perspectives. International Journal of Molecular Sciences. 2021; 22(21):11321. https://doi.org/10.3390/ijms222111321
