The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts

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dc.authoridDidem Karakaş Zeybek / 0000-0002-3781-6834en_US
dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidDidem Karakaş Zeybek / 56422040600
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidDidem Karakaş Zeybek / L-6682-2018
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorKarakaş Zeybek, Didem
dc.contributor.authorArı, Ferda
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2020-08-30T20:08:16Z
dc.date.available2020-08-30T20:08:16Z
dc.date.issued2017
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractThe MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plants are used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study, the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants (Hypericum adenotrichum, Salvia kronenburgii, and Pelargonium quercetorum) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231) using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to its interference-free feature. Additionally, decreased cell density was confirmed with phase-contrast microscopy and fluorescence staining (Hoechst 33342 dye). Although both of the viability/cytotoxicity assays are considered as metabolic assays, viabilities (in %) in the MTT assay were found to be strikingly higher when compared to the results with the ATP assay. Even in the case of total death, the MTT assay still produced artificial/false increases in viability. The morphology-based evaluation of viability/cytotoxicity by phase-contrast microscopy and Hoechst 33342 staining were greatly compatible with the ATP assay results. Overestimated (false) viabilities in the MTT assay suggests a serious interference between the MTT assay itself and the extracts used. Some ingredients of plants may have reducing activity (like the dehydrogenase activity of the cells) that converts the MTT compound into the colored formazan that is the principle of the assay. Therefore, the MTT assay may not be a suitable assay for some plant extracts, urging great caution when plants are used.en_US
dc.description.sponsorshipUludag University Scientific ResearchUludag University [BUAP(F)-2014/3]en_US
dc.description.sponsorshipThis study was supported by the Uludag University Scientific Research Projects Unit (BUAP(F)-2014/3 coded project).en_US
dc.identifier.citationKarakas, D., Ari, F., & Ulukaya, E. (2017). The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. TURKISH JOURNAL OF BIOLOGY, 41(6), 919–925. https://doi.org/10.3906/biy-1703-104en_US
dc.identifier.doi10.3906/biy-1703-104en_US
dc.identifier.endpage925en_US
dc.identifier.issn1300-0152en_US
dc.identifier.issn1303-6092en_US
dc.identifier.issue6en_US
dc.identifier.pmid30814856en_US
dc.identifier.scopus2-s2.0-85038415566en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage919en_US
dc.identifier.trdizinid255578en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1703-104
dc.identifier.urihttps://hdl.handle.net/20.500.12713/911
dc.identifier.volume41en_US
dc.identifier.wosWOS:000418253100007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarakaş Zeybek, Didemen_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMtt Assayen_US
dc.subjectAtp Assayen_US
dc.subjectInterferenceen_US
dc.subjectCytotoxicityen_US
dc.subjectPlant Extracten_US
dc.subjectBreast Canceren_US
dc.titleThe MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extractsen_US
dc.typeArticleen_US

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