Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer

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dc.authorscopusidNaveed Ahmed Khan / 7401797675
dc.authorscopusidRuqaiyyah Siddiqui / 18635417000
dc.authorwosidNaveed Ahmed Khan / FXL-5617-2022
dc.authorwosidRuqaiyyah Siddiqui / FVT-1496-2022
dc.contributor.authorHalim, Rohaya Abdul
dc.contributor.authorHalim, Hasseri
dc.contributor.authorHussain, Rosnani Hanim Mohd
dc.contributor.authorAazmi, Shafiq
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAnuar, Tengku Shahrul
dc.date.accessioned2025-06-03T13:18:10Z
dc.date.available2025-06-03T13:18:10Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractFree-living amoebae of the genus Acanthamoeba are causative agents of keratitis and amoebic encephalitis. They are widely found in various ecological environments. Therefore, the present study brings results that can help to better understand the genotypes of the environmental isolates and their pathogenicity. This study procured 26 Acanthamoeba isolates from three recreational lakes in 2022. Polymerase chain reaction amplification was performed on positive Acanthamoeba samples. The thermotolerance, osmotolerance, and cytopathogenicity in human keratinocyte (HaCaT) cells of the samples were also evaluated. The phylogenetic analysis demonstrated that 12 isolates were of genotype T4, two (T9), six (T17), four (T8), and one each from T5 and T11. The thermo- and osmotolerance assays indicated that eight Acanthamoeba samples were potentially pathogenic. Two T4 and one T9 genotype also recorded 33-, 42-, and 133-kDa serine-type proteases, respectively. The HaCaT cell monolayer revealed that three T4 and one T9 samples achieved cytopathic effects within the 50–100% range, hence significantly cytotoxic. The lactate dehydrogenase secretion results demonstrated that three (T4) and one (T9) sample exhibited exceptional toxicity (over 40%) compared to the other samples. The responses of Acanthamoeba members with similar genotypes to pathogenicity indicator assays varied considerably, rendering correlation of pathogenicity with specific genotypes challenging. © 2024 The Authors.
dc.description.sponsorshipThis study was financially supported by the Fundamental Research Grant Scheme (FRGS/1/2022/SKK0/UITM/02/13), Ministry of Higher Education, Malaysia and Geran Penyelidikan Penyelidik Muda Berbakat (600-RMC/YTR/5/3 [003/2021]), Universiti Teknologi MARA, Malaysia.
dc.identifier.citationHalim, R. A., Halim, H., Hussain, R. H. M., Aazmi, S., Khan, N. A., Siddiqui, R., & Anuar, T. S. (2024). Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer. Journal of Water and Health, 22(12), 2289-2303.
dc.identifier.doi10.2166/wh.2024.162
dc.identifier.endpage2303
dc.identifier.issn14778920
dc.identifier.issue12
dc.identifier.pmid39733356
dc.identifier.scopusqualityQ2
dc.identifier.startpage2289
dc.identifier.urihttp://dx.doi.org/10.2166/wh.2024.162
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7280
dc.identifier.volume22
dc.identifier.wosWOS:001369874100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.institutionauthoridNaveed Ahmed Khan / 0000-0001-7667-8553
dc.institutionauthoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.language.isoen
dc.publisherIWA Publishing
dc.relation.ispartofJournal of Water and Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcanthamoeba
dc.subjectCytopathic Effect
dc.subjectMalaysia
dc.subjectPathogenicity
dc.subjectProtease
dc.titleAssessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer
dc.typeArticle

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