Combination of high performance liquid chromatography and flame atomic absorption spectrophotometry using a novel nebulizer interface supported T shaped slotted quartz tube for the determination of Vitamin B12

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dc.authoridSüleyman Bodur / 0000-0002-2994-9047en_US
dc.authorscopusidSüleyman Bodur / 57202493954
dc.authorwosidSüleyman Bodur / AHB-4581-2022en_US
dc.contributor.authorErarpat, Sezin
dc.contributor.authorBodur, Süleyman
dc.contributor.authorGünkara, Ömer Tahir
dc.contributor.authorBakırdere, Sezgin
dc.date.accessioned2022-06-03T14:16:10Z
dc.date.available2022-06-03T14:16:10Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Temel Bilimleri Bölümüen_US
dc.description.abstractA novel nebulizer interface (NI) was proposed to combine high performance liquid chromatography (HPLC) and flame atomic absorption spectrophotometer (FAAS). A glass concentric nebulizer was linked to T-shaped slotted quartz tube (T-SQT) using a tubing to transfer the liquid solution eluted from the chromatographic system into the atomization region of FAAS system. T-SQT was also used to intensify the interaction of atoms with the hollow cathode lamp light. Vitamin B12 was selected as an analyte to show the applicability of the new hyphenated system. After optimizing some parameters such as mobile phase flow rate and pH, nebulizer gas flow rate, T-SQT height and injection volume, linear range for the analyte was determined between 4.7 and 92 mg/kg as Co. Limit of detection (LOD) and limit of quantitation (LOQ) for the HPLC-NI-T-SQT-FAAS system were calculated to be 1.6 and 5.3 mg/kg as Co, respectively. Recovery studies were also conducted to verify the accuracy and applicability of the developed method for vitamin tablets and excellent percent recovery results (~ 100%) with low standard deviation values were obtained when matrix-matching calibration strategy was performed for each vitamin tablet. A successful separation and detection of the analyte was achieved within 3.0 min that offers high sample throughput. Two different vitamin tablets were analyzed by the optimized hyphenated system. The developed method also provides low usage of sample solution in contrast to conventional nebulizer in the FAAS systemen_US
dc.identifier.citationErarpat S, Bodur S, Günkara ÖT, Bakırdere S. Combination of high performance liquid chromatography and flame atomic absorption spectrophotometry using a novel nebulizer interface supported T shaped slotted quartz tube for the determination of Vitamin B12. J Pharm Biomed Anal. 2022 May 23;217:114855. doi: 10.1016/j.jpba.2022.114855. Epub ahead of print. PMID: 35636012.en_US
dc.identifier.doi10.1016/j.jpba.2022.114855en_US
dc.identifier.issn0731-7085en_US
dc.identifier.pmid35636012en_US
dc.identifier.scopus2-s2.0-85131062143en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttp://doi.org/ 10.1016/j.jpba.2022.114855
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2795
dc.identifier.volume217en_US
dc.identifier.wosWOS:000806857900002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBodur, Süleyman
dc.language.isoenen_US
dc.publisherElsevier Scienceen_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHigh Performance Liquid Chromatographyen_US
dc.subjectFlame Atomic Absorption Spectrophotometryen_US
dc.subjectConcentric Nebulizeren_US
dc.subjectT-shaped Slotted Quartz Tubeen_US
dc.titleCombination of high performance liquid chromatography and flame atomic absorption spectrophotometry using a novel nebulizer interface supported T shaped slotted quartz tube for the determination of Vitamin B12en_US
dc.typeArticleen_US

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