Increased levels of nerve growth factor accompany oxidative load in recurrent pregnancy loss. machine learning applied to FT-raman spectra study

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dc.authoridHuri Bulut / 0000-0003-2706-9625en_US
dc.authorscopusidHuri Bulut / 57185264300en_US
dc.authorwosidHuri Bulut / CGN-9704-2022en_US
dc.contributor.authorDepciuch, Joanna
dc.contributor.authorJakubczyk, Pawel
dc.contributor.authorPaja, Wieslaw
dc.contributor.authorPancerz, Krzysztof
dc.contributor.authorWosiak, Agnieszka
dc.contributor.authorBahat, Pinar Yalcin
dc.contributor.authorToto, Omer Faruk
dc.contributor.authorBulut, Huri
dc.contributor.authorGuleken, Zozan
dc.date.accessioned2023-08-03T11:07:55Z
dc.date.available2023-08-03T11:07:55Z
dc.date.issued2023en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractThe presented article is focused on developing and validating an efficient, credible, minimally invasive technique based on spectral signatures of blood serum samples in patients with diagnosed recurrent pregnancy loss (RPL) versus healthy individuals who were followed at the Gynecology department. A total of 120 participants, RPL disease (n = 60) and healthy individuals (n = 60), participated in the study. First, we investigated the effect of circulating nerve growth factor (NGF) in RPL and healthy groups. To show NGF's effect, we measured the level of oxidative loads such as Total Antioxidant Level (TAS), Total Oxidant Level (TOS), and Oxidative Stress Index (OSI) with Beckman Coulter AU system and biochemical assays. We find a correlation between oxidative load and NGF level. Oxidative load mainly causes structural changes in the blood. Therefore, we obtained Raman measurements of the participant's serum. Then we selected two Raman regions, 800 and 1800 cm(-1), and between 2700 cm(-1) and 3000 cm(-1), to see chemical changes. We noted that Raman spectra obtained for RPL and healthy women differed. The findings confirm that the imbalance between reactive oxygen species and antioxidants has important implications for the pathogenesis of RPL and that NGF levels accompany the level of oxidative load in the RPL state. Biomolecular structure and composition were determined using Raman spectroscopy and machine learning methods, and the correlation of these parameters was studied alongside machine learning technologies to advance toward clinical translation. Here we determined and validated the development of instrumentation for the Analysis of RPL patients' serum that can differentiate from control individuals with an accuracy of 100% using the Raman region corresponding to structural changes. Furthermore, this study found a correlation between traditional biochemical parameters and Raman data. This suggests that Raman spectroscopy is a sensitive tool for detecting biochemical changes in serum caused by RPL or other diseases.en_US
dc.identifier.citationDepciuch, J., Jakubczyk, P., Paja, W., Pancerz, K., Wosiak, A., Bahat, P. Y., ... & Guleken, Z. (2023). Increased levels of nerve growth factor accompany oxidative load in recurrent pregnancy loss. Machine learning applied to FT-Raman spectra study. Bioprocess and Biosystems Engineering, 599-609.en_US
dc.identifier.doi10.1007/s00449-023-02847-8en_US
dc.identifier.endpage609en_US
dc.identifier.issn1615-7591en_US
dc.identifier.issn1615-7605en_US
dc.identifier.issue4en_US
dc.identifier.pmid36702951en_US
dc.identifier.scopus2-s2.0-85146881987en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage599en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s00449-023-02847-8
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3952
dc.identifier.volume46en_US
dc.identifier.wosWOS:000918507500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBulut, Huri
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBioprocess and Biosystems Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRecurrent Pregnancy Lossen_US
dc.subjectNerve Growth Factoren_US
dc.subjectOxidative Stress Indexen_US
dc.subjectRaman Spectroscopyen_US
dc.subjectMachine Learningen_US
dc.titleIncreased levels of nerve growth factor accompany oxidative load in recurrent pregnancy loss. machine learning applied to FT-raman spectra studyen_US
dc.typeArticleen_US

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