Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7-s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3, NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells. Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells. Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer.
Açıklama
Anahtar Kelimeler
Erturk, Elif, Ari, Ferda, Akgun, Oguzhan, Ulukaya, Engin, Kucukali, Cem Ismail, Zeybek, Umit
Kaynak
TURKISH JOURNAL OF BIOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
45
Sayı
5
Künye
ERTÜRK, E., ARI, F., AKGÜN, O., ULUKAYA, E., KÜÇÜKALİ, C. İ., & ZEYBEK, Ş. Ü. (2021). Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells. Turkish Journal of Biology, 45(5), 613-623.
