Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells

dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorErtürk, Elif
dc.contributor.authorArı, Ferda
dc.contributor.authorAkgün, Oguzhan
dc.contributor.authorUlukaya, Engin
dc.contributor.authorKüçükali, Cem İsmail
dc.contributor.authorZeybek, Ümit
dc.date.accessioned2021-11-02T08:42:34Z
dc.date.available2021-11-02T08:42:34Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractUnderstanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7-s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3, NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells. Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells. Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer.en_US
dc.identifier.citationERTÜRK, E., ARI, F., AKGÜN, O., ULUKAYA, E., KÜÇÜKALİ, C. İ., & ZEYBEK, Ş. Ü. (2021). Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells. Turkish Journal of Biology, 45(5), 613-623.en_US
dc.identifier.doi10.3906/biy-2103-46en_US
dc.identifier.endpage+en_US
dc.identifier.issn1300-0152en_US
dc.identifier.issn1303-6092en_US
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85118206781en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage613en_US
dc.identifier.trdizinid514970en_US
dc.identifier.urihttps://doi.org/10.3906/biy-2103-46
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2198
dc.identifier.volume45en_US
dc.identifier.wosWOS:000708974500003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectErturk, Elifen_US
dc.subjectAri, Ferdaen_US
dc.subjectAkgun, Oguzhanen_US
dc.subjectUlukaya, Enginen_US
dc.subjectKucukali, Cem Ismailen_US
dc.subjectZeybek, Umiten_US
dc.titleInvestigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cellsen_US
dc.typeArticleen_US

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