Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells
dc.authorid | Engin Ulukaya / 0000-0003-4875-5472 | en_US |
dc.authorscopusid | Engin Ulukaya / 6602927353 | |
dc.authorwosid | Engin Ulukaya / K-5792-2018 | |
dc.contributor.author | Ertürk, Elif | |
dc.contributor.author | Arı, Ferda | |
dc.contributor.author | Akgün, Oguzhan | |
dc.contributor.author | Ulukaya, Engin | |
dc.contributor.author | Küçükali, Cem İsmail | |
dc.contributor.author | Zeybek, Ümit | |
dc.date.accessioned | 2021-11-02T08:42:34Z | |
dc.date.available | 2021-11-02T08:42:34Z | |
dc.date.issued | 2021 | en_US |
dc.department | İstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
dc.description.abstract | Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7-s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3, NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells. Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells. Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer. | en_US |
dc.identifier.citation | ERTÜRK, E., ARI, F., AKGÜN, O., ULUKAYA, E., KÜÇÜKALİ, C. İ., & ZEYBEK, Ş. Ü. (2021). Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells. Turkish Journal of Biology, 45(5), 613-623. | en_US |
dc.identifier.doi | 10.3906/biy-2103-46 | en_US |
dc.identifier.endpage | + | en_US |
dc.identifier.issn | 1300-0152 | en_US |
dc.identifier.issn | 1303-6092 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.scopus | 2-s2.0-85118206781 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.startpage | 613 | en_US |
dc.identifier.trdizinid | 514970 | en_US |
dc.identifier.uri | https://doi.org/10.3906/biy-2103-46 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12713/2198 | |
dc.identifier.volume | 45 | en_US |
dc.identifier.wos | WOS:000708974500003 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | TR-Dizin | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.institutionauthor | Ulukaya, Engin | |
dc.language.iso | en | en_US |
dc.publisher | TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY | en_US |
dc.relation.ispartof | TURKISH JOURNAL OF BIOLOGY | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Erturk, Elif | en_US |
dc.subject | Ari, Ferda | en_US |
dc.subject | Akgun, Oguzhan | en_US |
dc.subject | Ulukaya, Engin | en_US |
dc.subject | Kucukali, Cem Ismail | en_US |
dc.subject | Zeybek, Umit | en_US |
dc.title | Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells | en_US |
dc.type | Article | en_US |