Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype

dc.authorscopusidRuqaiyyah Siddiqui / 18635417000
dc.authorscopusidNaveed Ahmed Khan / 7401797675
dc.authorwosidRuqaiyyah Siddiqui / FVT-1496-2022
dc.authorwosidNaveed Ahmed Khan / FXL-5617-2022
dc.contributor.authorAkbar, Noor
dc.contributor.authorKhan, Amir Sada
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorIbrahim, Taleb Hassan
dc.contributor.authorKhamis, Mustafa I.
dc.contributor.authorAlawfi, Bader S.
dc.contributor.authorAl-ahmadi, Bassam M.
dc.contributor.authorKhan, Naveed Ahmed
dc.date.accessioned2025-04-18T10:12:17Z
dc.date.available2025-04-18T10:12:17Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractHerein, we investigated the anti-amoebic activity of phosphonium-chloride-based deep eutectic solvents against pathogenic Acanthamoeba castellanii of the T4 genotype. Deep eutectic solvents are ionic fluids composed of two or three substances, capable of self-association to form a eutectic mixture with a melting point lower than each substance. In this study, three distinct hydrophobic deep eutectic solvents were formulated, employing trihexyltetradecylphosphonium chloride as the hydrogen bond acceptor and aspirin, dodecanoic acid, and 4-tert-butylbenzoic acid as the hydrogen bond donors. Subsequently, all three deep eutectic solvents, denoted as DES1, DES2, DES3 formulations, underwent investigations comprising amoebicidal, adhesion, excystation, cytotoxicity, and cytopathogenicity assays. The findings revealed that DES2 was the most potent anti-amoebic agent, with a 94% elimination rate against the amoebae within 24 h at 30 degrees C. Adhesion assays revealed that deep eutectic solvents hindered amoebae adhesion to human brain endothelial cells, with DES2 exhibiting 88% reduction of adhesion. Notably, DES3 exhibited remarkable anti-excystation properties, preventing 94% of cysts from reverting to trophozoites. In cytopathogenicity experiments, deep eutectic solvent formulations and dodecanoic acid alone reduced amoebae-induced human brain endothelial cell death, with DES2 showing the highest effects. Lactate dehydrogenase assays revealed the minimal cytotoxicity of the tested deep eutectic solvents, with the exception of trihexyltetradecylphosphonium chloride, which exhibited 35% endothelial cell damage. These findings underscore the potential of specific deep eutectic solvents in combating pathogenic Acanthamoeba, presenting promising avenues for further research and development against free-living amoebae.
dc.description.sponsorshipUnited States Department of Defense Air Force Office of Scientific Research (AFOSR)
dc.identifier.citationAkbar, N., Khan, A. S., Siddiqui, R., Ibrahim, T. H., Khamis, M. I., Alawfi, B. S., ... & Khan, N. A. (2024). Phosphonium chloride–based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype. Folia Microbiologica, 1-13.
dc.identifier.doi10.1007/s12223-024-01180-1
dc.identifier.issn0015-5632
dc.identifier.issn1874-9356
dc.identifier.pmid38869777
dc.identifier.scopus2-s2.0-85195789513
dc.identifier.scopusqualityQ2
dc.identifier.urihttp://dx.doi.org/10.1007/s12223-024-01180-1
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6990
dc.identifier.wosWOS:001247195100002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.institutionauthoridNaveed Khan / 0000-0001-7667-8553
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofFolia microbiologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcanthamoeba Castellanii
dc.subjectCytotoxicity
dc.subjectDeep Eutectic Solvents
dc.subjectExcystment
dc.subjectLactate Dehydrogenase
dc.titlePhosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype
dc.typeArticle

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